Study on Isolation, Purification and Identification of Glycoprtein from Chinese Yam

FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (7): 217-220.

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Study on Isolation, Purification and Identification of Glycoprtein from Chinese Yam

DING Qing-Zhi, MA Hai-Le, LUO Lin, LI Jin-Zhong

1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2.Jiangsu Province Research Center of Bio-process and Separation of Agri-products, Zhenjiang 212013, China

Online:2008-07-15 Published:2011-07-28

Abstract

Abstract: Glycoprtein from Chinese yam purified by DEAE-cellulose 52. It was successful to obtain one fraction GLP by the step gradient elution. GLP was further purified by Sephadex G-75. TLC was applied to identify the purity of the GLP. The molecular weight of GLP measured by Sephadex G-75 is 89381 D. The experimental result of TLC revealed that the monosac-charide components of GLP are L-galactose, D-glucose and D-mannose. GLP is confirmed by its characteristic absorption peak of infrared spectrum.

Key words: Chinese yam, glycoprtein, purification, character

DING Qing-Zhi, MA Hai-Le, LUO Lin, LI Jin-Zhong. Study on Isolation, Purification and Identification of Glycoprtein from Chinese Yam[J]. FOOD SCIENCE, 2008, 29(7): 217-220 https://doi.org/

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Study on Isolation, Purification and Identification of Glycoprtein from Chinese Yam

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