Study on Enzymatic Modification of Steviosides

Abstract

Abstract: This study aimed to improve lingering bitterness of steviosides by enzymatically modifying steviosides with α-amylase and cyclodextrin glucanotransferase(CGTase) and to optimize the parameters of enzymatic modification process. The obtained process flow is starch hydrolysis → enzymatic modification → decoloration → purification with resin → spraydrying,where the enzymatic modification is the key step. The parameters in the above flow were investigated respectively,including concentration of starch,temperature,pH,enzymes amounts and ratio of starch to steviosides.

Key words: steviosides； enzymatic modification； cyclodextrin glucanotransferase(CGTase)； &alpha, -amylase

YU Jun, YUE Peng-Xiang, CHENG Qi-Chun. Study on Enzymatic Modification of Steviosides[J]. FOOD SCIENCE, 2008, 29(8): 214-218 https://doi.org/

[1]	LIU Xiaoze, ZHANG Ganqi, SUN Ruoziying, LIU Yingxin, WANG Lu. Inhibitory Mechanism of Characteristic Polyphenols in Hainan Large-Leaf Tea against Carbohydrate Hydrolases [J]. FOOD SCIENCE, 2026, 47(10): 39-54.
[2]	LAO Yulu, ZHENG Qinsheng, ZHAO Kaixin, XIAO Jie, CAO Yong, LIU Xiaojuan. Interaction of Astaxanthin Stereoisomers with Porcine Pancreatic α-Amylase [J]. FOOD SCIENCE, 2025, 46(17): 31-42.
[3]	DONG Shihao, XU Ningmeng, FU Zhengjian, SHEN Kaize, ZENG Shunchao, FAN Fangyu, GUO Lei. Identification of Polyphenolic Components in Gastrodia elata and Their Inhibitory Effects on α-Amylase and α-Glucosidase [J]. FOOD SCIENCE, 2025, 46(16): 285-294.
[4]	ZOU Xiaojun, ZHENG Qinsheng, ZHAO Kaixin, FENG Yujie, XIAO Jie, HE Liping, CAO Yong, LIU Xiaojuan. Differences in Inhibitory Effects and Mechanism of All-E and Z-Astaxanthin on Pancreatic α-Amylase [J]. FOOD SCIENCE, 2025, 46(15): 35-46.
[5]	WANG Qian, WANG Juan, FU Jinfeng, SHENG Ou. Identification of Polyphenolic Components in Unripe Banana Pulp and Their Inhibitory Effects on α-Amylase and α-Glucosidase [J]. FOOD SCIENCE, 2024, 45(7): 61-68.
[6]	HUANG Jingjing, ZHOU Yingqin, LUO Zhang, LIU Zhendong, CHENG Xiufeng, XIE Ningning. Isolation, Purification, Identification and Quantum Chemical Characterization of Blood Glucose-Regulating Peptides Derived from Dry-Cured Ham of Wanzhe Spotted Pigs [J]. FOOD SCIENCE, 2024, 45(3): 25-33.
[7]	LI Yongfu, WANG Yaru, HUANG Jinrong, SHI Feng. Preparation and Identification of α-Amylase Inhibitory Peptides from Mung Bean Protein [J]. FOOD SCIENCE, 2024, 45(1): 58-64.
[8]	SHEN Heyu, LI Mengyang, AO Jingfang, WANG Jun, XU Huaide, LUO Anwei. Inhibitory Effect and Mechanism of Gallic Acid on α-Amylase and α-Glucosidase Activities [J]. FOOD SCIENCE, 2023, 44(22): 31-38.
[9]	LI Zhipeng, ZHOU Chenyi, PAN Shutong, CUI Zewen, XIE Fan, WANG Guangqiang, AI Lianzhong, ZHANG Hui. Inhibitory Effects of Two Polysaccharides from Highland Barley on the Activity of Pancreatic α-Amylase [J]. FOOD SCIENCE, 2023, 44(22): 192-199.
[10]	KUANG Jiwei, ZHANG Chong, HUANG Junrong, PU Huayin, MA Wenhui, MIN Cong, Youling L. XIONG. Mechanism of the Influence of Different Gluten Fractions on the Digestibility of Wheat Starch [J]. FOOD SCIENCE, 2023, 44(2): 18-25.
[11]	LI Mengyao, LIANG Qi, SONG Xuemei. Using Molecular Docking to Investigate the Alpha-amylase Inhibitory Activity of Bitter Peptides RK7 and KQ7 Derived from Yak Cheese [J]. FOOD SCIENCE, 2023, 44(2): 132-138.
[12]	WANG Wei, CUI Yan, ZHENG Mingzhu, CAI Dan, LIU Jingsheng, LIU Meihong, LIU Huimin. Inhibitory Kinetics of Cyanidin-3-O-glucoside against α-Amylase and α-Glucosidase [J]. FOOD SCIENCE, 2023, 44(16): 185-191.
[13]	ZHANG Yan, SUN Juan, BAO Feifei, DENG Yuanyuan, LIU Pei, HUO Junwei,. Identification and Bioactivity Analysis of Polyphenols in Blue Honeysuckle Fruit from Different Regions of the Greater Khingan Mountains [J]. FOOD SCIENCE, 2023, 44(12): 225-234.
[14]	ZHANG Ke, WANG Shiguang, SONG Yanyan, LI Peigang, WANG Bing, XUE Wentong. Effect of Direct Extrusion Modification on the Structure and Nutritional Properties of Rice Analogues [J]. FOOD SCIENCE, 2023, 44(11): 72-78.
[15]	ZHAO Shuting, WANG Weihao, QUAN Zhigang, WANG Juan, LIU Dezhi, WANG Yifei, WU Yunjiao, SU Youtao, WEI Chunhong, CAO Longkui. Preparation and Structural Characterization of Mung Bean RS4·Se (IV) and Kinetics of Enzymatic Inhibition by It [J]. FOOD SCIENCE, 2022, 43(20): 53-62.

Study on Enzymatic Modification of Steviosides

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments