Abstract: A method for the determination of vitamin B1 (VB1) in millet which is one of important grain in china by high performance liquid chromatography (HPLC) was developed. After extracting by hydrochloric acid and taka diastase, the enzymatically treated sample can separated from fecula, fatty compound and protein and so on in millet completely. A VP-ODS C18 column was used for separation and elution was performed with methanol and sodium acetate (0.5 mol/L, pH4.5, 20:80). VB1 was detected by fluorescence detector quantificationally. The average recoveries were 91.59%～94.27% of high, middle and low three level (n=4)in this method. The relative standard deviations(RSD%) were 2.8%～5.9%(n=6). The detection limit (S/N=3) was 1.56 ng/ml.

Key words: VB1, millet, HPLC