Abstract: Colloidal gold was prepared with reducing reaction of sodium citrate and was coulped with monocolonal anti-Sulfadiazine in this article. BSA-sulfadiazine was coupled with the nitrocellulose membranes to prepare immunochromatographic strips. With this method, semi-quantitative detection of sulfadiazine can be finished in less than 15 min. The sensitivity to sulfadiazine is 5 ng/ml, and no cross-reactivity with other sulfonamides. Good recovery rates are determined from 71% to 95% in chickens meats. Based on maximum residue level of sulfadiazine (100 ng/g) in China, this method was compared with with high-performance liquid chromatographic (HPLC) by assaying 56 chicken meat samples taken from animal experiment. The agreement rates are 100%. This method is a sensitive, simple, rapid assay without the need of special apparatus, so it is of good practical value.

Key words: colloidal gold, immunochromatography, chicken meats, sulfadiazine