Abstract: Clenbuterol is a β-agonist used as a therapeutic agent in medicine. At high concentrations clenbuterol promotes the growth of farm animals but its use as a growth promoter is prohibited and its misuse has been related to food poisoning as results of consuming contaminated meat. Traditional methods for detection of clenbuterol often require elaborate sample preparation procedures and involve tedious time consuming protocols. So developing a new immuno-biosensor method for capture and detection such small molecular is still a big challenge. This research constructed an immuno-rotary biosensor (IRB) for clenbuterol detecting, which based on the fluorescent technique and BPCL (bilogical and chemical photon counter luminescene). The results showed that a hypersensitive biosensor for quickly detection of clenbuterol was achieved.

Key words: clenbuterol hydrochloride, quantumdots, ELISA, ATP, immuno-rotary biosensor, BPCL