Research on Protoplast Formation and Regeneration of Two Yeasts Isolated from Highland Barley Wine Starters

Abstract

Abstract: The optimum conditions for preparation and regeneration of ZY2 and ZY8 protoplasts were obtained by studying the effects of different factors on them as following.Treating 10-hour culture of strain ZY2 or ZY8 in logarithmic growth phase with concentration of 1.5% snailase or 1.5% snailase and 1.0% lywallzyme for 1 hour,after pretreatment on the cells with 0.1% β-mercaploethanol or together treated with 20 mmol/L DTT.With 0.7 mol/L KC1 as osmotic stabilizer during the protoplast formation and 17% sucrose for the regeneration,the protoplast formation is 94.15% and 88.78%,and regeneration ratio reaches 13.85% and 23.02% respectively.

Key words: highland barley wine starter, Saccharomyces cerevisiae, Candida sp., protoplast formation, protoplast regeneration

DU Mu-Ying, CHEN Juan, HAN Jian-Quan, BATA-VIDACS Ildiko, BECZNER Judit, CHEN Zong-Dao. Research on Protoplast Formation and Regeneration of Two Yeasts Isolated from Highland Barley Wine Starters[J]. FOOD SCIENCE, 2007, 28(9): 378-383 https://doi.org/

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Research on Protoplast Formation and Regeneration of Two Yeasts Isolated from Highland Barley Wine Starters

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