Abstract: EDC method and GA method were used to conjugate Oxytetracycline to Bovine Serum albumin(BSA), then artificial antigen, Oxytetracycline and BSA were respectively taken on UV spectrum scan. The UV spectrum of artificial antigen has been changed when compared to BSA and Oxytetracycline. The result showed that artificial antigen is synthesized successfully. The molar ratio of Oxytetracycline to BSA in artificial antigen was 7.97:1 (using EDC method ) and 2.85:1 (GA method), this means that using different conjugative compound will get different artificial antigen. BALB/c small mice had been immunized with Oxytetracycline-BSA for 65 days, and the titer antiserum from those mice were testified by indirect ELISA, the titer of the antiserum is 1:20000.

Key words: Oxytetracycline, artificiad antige, antiserum, immunological analysis