Abstract:

Objective: The artificial antigens were synthesized by derivative of clopidol and BSA、OVA.Methods: The derivative of clopidol, called as CLOP-2C, was synthesized by Williamson method in this study. Then two artificial antigens of clopidol(CLOP-2C-BSA, CLOP-2C-OVA) were prepared by active ester method (AE) and mixed anhydride method (MA) respectively. Results: CLOP-2C is identified by TLC, MS, IR spectrum . The characterization of hapten-protein conjugates were examined by ultraviolet spectrophotometry. The numbers of hapten molecules per molecule of protein estimated by ultraviolet spectrophotometry according to the absorbance of compounds at 280 nm for two conjugates were 8.26 and 4.7 respectively Conclusion: The artificial antigens were synthesized successful.

Key words: clopidol, hapten, carrier-protein, artificial antigen