Abstract:

In this study, the hapten of tenuazonic acid, the most harmful Alternaria mycotoxin, was designed and modified.
We systematically investigated the selection of carrier protein and coupling method of hapten derivatives for the carrier,
the purification and identification of artificial antigens, and the analysis of various factors that affect the synthesis of
immunogens to determine tenuazonic acid coupling with BSA protein by EDC method. An artificial antigen was obtained
successfully with a coupling ratio of 9.7:1, which could be used to immunize animals.

Key words: tenuazonic acid, artificial antigen, carbodiimide