Study on Determination of Phenolic Acid in Sunflower Seeds by HPLC

Abstract

Abstract: A method for determination phenolic acid(chlorogenic acid and caffeic acid)in sunflower seeds by HPLC was described.Chromatographi c separation was performed ALLTIMA C 18 column (250mm ×4.6mm ID,5μm)byusingan acetonitrile -2%glacicalaceticacid(15∶85,v /v)mixtureas the mobilephase andusing SPD -6AVultraviolet detection at 327nmwith a flowrate of l.0ml /min.The chlorogenic acid and caffeic acid were eluted in 18min with satisfacto ry separation and the content of phen olic acid in different genotypes of sun-flower was also determinded.

Key words: HPLC, sunflower seed, phenolic acid

CHEN Shao-Zhou, LU Fei-Jie , TAI Jian-Xiang. Study on Determination of Phenolic Acid in Sunflower Seeds by HPLC[J]. FOOD SCIENCE, 2003, 24(1): 107-110.

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Study on Determination of Phenolic Acid in Sunflower Seeds by HPLC

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