Orthogonal Array Design for the Optimization of Successive Extraction of Total Flavonoids and Polysaccharides from Epimedium brevicornum Maxim

doi:10.7506/spkx1002-6630-201224012

Abstract

Abstract:

Objective: To optimize process conditions for the extraction of total flavonoids from the whole plant of Epimedium brevicornum Maxim and for further extraction of polysaccharides from the remaining residue. Methods: A two-step sequential extraction procedure was proposed for the extraction of total flavonoids by ultrasonic-assisted extraction and polysaccharides by hot water extraction. Results: The optimum conditions for the extraction of total flavonoids were found to be two extraction cycles at 60 ℃ and a microwave power of 150 W for 5 min each cycle with 70% ethanol at a solid-to-solvent ratio of 1:10 (g/mL), resulting in an extraction efficiency of 4.72%. The optimum conditions for the extraction of polysaccharides were found to be two extraction cycles at 90 ℃ for 30 min each cycle with a residue-to-water ratio of 1:10 g/mL, resulting in an extraction efficiency of 1.89%. The optimized procedure for sequential extraction of total flavonoids and polysaccharides was characterized by easy operation, low energy consumption, high extraction efficiency, low polysaccharide loss and enhanced comprehensive utilization of Epimedium brevicornum Maxim.

Key words: Epimedium brevicornum Maxim, total flavonoids, polysaccharides, two-step extraction

CLC Number:

R284.2

FU Liang1，YUAN Jingya2，YANG Rui-wu1，ZHANG Li1，ZHOU Yong-hong2，DING Chun-bang1,*. Orthogonal Array Design for the Optimization of Successive Extraction of Total Flavonoids and Polysaccharides from Epimedium brevicornum Maxim[J]. FOOD SCIENCE, 2012, 33(24): 56-60.

References

[1] 李时珍. 本草纲目[M]. 北京: 人民卫生出版社, 1982: 750

[2]Lu Y, Wang D, Hu Y, et al.Sulfated modification of epimedium polysaccharide and effects of the modifiers on cellular infectivity of IBDV[J].Carbohydr Polym,2008,71:180-186

[3]Kong X, Hu Y, Rui R, et al.Effects of Chinese herbal medicinal ingredients on peripheral lymphoctye proliferation and serum antibody titer after vaccination in chicken[J].Int Immunopharmacol,2004,4:975-982

[4]Wang D, Li X, Xu L, et al.Immunologic synergism with IL-2 and effects of cCHMIs on mRNA expression of IL-2 and IFN-γ in chicken peripheral T lymphocyte[J].Vaccine,2006,24:7109-7114

[5]郭宝林, 罗崇念, 肖培根.淫羊藿多糖研究进展[J].中国中药杂志,1998,23(7):436-437

[6] 付亮, 杨瑞武, 张利, 等. 淫羊藿多糖的研究进展及开发前景[J].食品科学,,:-

[7]Yang H, Chen D, Cui Q C, et al.Celastrol, a triterpene extracted from the Chinese “Thunder of God Vine,” is a potentproteasome inhibitor and suppresses human prostate cancer growth in nude mice[J].Cancer Res,2006,66(9):4758-4765

[8]张华峰, 杨晓华.淫羊藿的生物活性成分及其开发策略研究[J].中草药,2010,41(2):329-332

[9]陈良柱, 杨利军, 时书宁.淫羊藿药理学研究进展[J].动物医学进展,2008,29(增):33-34

[10]李花.淫羊藿药理作用的研究现状及展望[J].现代医药卫生,2008,24(22):3413-3414

[11]张华峰, 杨晓华.淫羊藿黄酮类化合物提取研究进展[J].中药材,2010,33(3):470-474

[12]杨晓华, 张华峰, 王瑛.淫羊藿多糖的功能、提取及开发前景[J].中国食品添加剂,2008,S1:184-187

[13]陈鸿英, 朱永智, 吴乃居, 等.朝鲜淫羊藿多糖的含量测定[J].中草药,2003,34(9):810-811

[14] 国家药典委员会. 中国药典(一部)[M]. 北京: 化学工业出版社, 2010: 306

[15]郑晓翠, 苏瑛, 唐咏, 等.淫羊藿多糖的提取及其蛋白的脱除[J].华西药学杂志,2009,24(2):155-157

[16]罗燕, 邵永斌, 谷新利, 等.淫羊藿多糖提取工艺的优化及对小鼠细胞免疫功能的影响[J].动物保健品,2009,5:104-106

[17]苏瑛, 郑晓翠, 唐咏, 等.淫羊藿多糖的酶法提取及其抗氧化作用的研究[J].食品科技(提取物与应用,2009,34(4):200-203

[18]Wei X L, Chen M A, Xiao J B, et al.Composition and bioactivity of tea flower polysaccharides obtained by different methods[J].Carbohydrate Polymers,2010,79(2):418-422