Abstract:

A method for the determination of glucose, fructose and sucrose in royal jelly using ion chromatography
coupled with online dialysis following protein precipitation with trichloroacetic acid has been developed. The optimal
chromatographic and dialysis conditions were investigated. The instrument consisted of a METROSEP CARB 1(150 mm ×
4.0 mm) anion exchange column and a pulsed amperometric detector. Elutions were performed using 100.0 mmol/L NaOH
at a flow rate of 1.0 mL/min. Dialysis time and transfer time were 10 min and 33 s, respectively. The detection limits for
glucose, fructose and sucrose were 0.068, 0.085 mg/L and 0.081 mg/L, respectively. The recoveries were 85%–107%. This
method proved suitable for the simultaneous determination of glucose, fructose and sucrose in royal jelly.

Key words: ion chromatography, pulsed amperometric detection, dialysis, glucose, fructose, sucrose