Effect of a Polysaccharide from Ganoderma atrum on cAMP/PKA, IP3/Ca2+ and DAG/PKC Signaling Pathways in Peritoneal Macrophages of S-180 Tumor-Bearing Mice

doi:10.7506/spkx1002-6630-201401040

Abstract

Abstract:

Objective: To explore the effects of a polysaccharide from Ganoderma atrum (PSG-1) on cyclic adenosine
monophosphate (cAMP)/protein kinase A (PKA), inositol triphosphate (IP3)/Ca2+ and diacylglycerol (DAG)/protein kinase C
(PKC) signaling pathways in peritoneal macrophages of S-180 tumor-bearing mice. Methods: A tumor-bearing mouse model
was established by inoculating mouse sarcoma S-180 cells into BALB/c mice; peritoneal macrophages were collected from
the S-180 tumor-bearing mice, then cultured in vitro and treated with PSG-1 at various concentrations. The IP3, DAG and
cAMP levels in cell culture supernatant were measured by ELISA. The intracellular Ca2+ content was assayed by a flow
cytometric method. The PKA and PKC protein expression in macrophages was determined by Western blotting. Results:
PSG-1 in the concentration range of 20–160 μg/mL stimulated the production of IP3, DAG and cAMP in the macrophages
from S-180 tumor-bearing mice, increased the intracellular Ca2+ content, and increased the protein expression of PKA and
PKC. Conclusion: PSG-1 can exert anti-tumor activity in peritoneal macrophages of S-180 tumor-bearing mice through the
activation of cAMP/PKA, IP3/Ca2+ and DAG/PKC signaling pathways.

Key words: polysaccharides from Ganoderma atrum, peritoneal macrophages, anti-tumor activity, signaling pathway

CLC Number:

Q291

HUANG Jian-qin, NIE Shao-ping*, ZHANG Shen-shen, HUANG Dan-fei, ZHU Ke-xue, XIE Ming-yong. Effect of a Polysaccharide from Ganoderma atrum on cAMP/PKA, IP3/Ca2+ and DAG/PKC Signaling Pathways in Peritoneal Macrophages of S-180 Tumor-Bearing Mice[J]. FOOD SCIENCE, 2014, 35(1): 204-208.

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