Abstract:

A method for rapid determination of 8 ginsenosides in ginseng extracts of different sources using ultra
performance liquid chromatography (UPLC) was established. The content of ginsenosides in 44 batches of ginseng extracts
from 13 sources was analyzed and evaluated. The separation was performed on a Waters ACQUITY UPLC BEH® C18
column (50 mm×2.1 mm, 1.7 μm) at 30 ℃ at a flow rate of 0.5 mL/min through gradient elution with acetonitrile-water
mixture and detection at a wavelength of 203 nm. Eight ginsenosides were well separated within 15 min with correlation
coefficients of 0.9990–0.9997. The detection limits for ginsenosides were 0.47-1.96 ng with recoveries ranging from 95.0%
to 110.0%. The method was simple, rapid, and reliable, and thus, was suitable for the rapid determination of 8 ginsenosides
in ginseng extracts from different sources.

Key words: ultra performance liquid chromatography (UPLC), ginseng extracts, ginsenosides