Effects of pH and Substrate Feeding on Phenyllactic Acid Production by Lactobacillus paracasei W2

doi:10.7506/spkx1002-6630-201401032

Abstract

Abstract:

In this paper, the effects of initial medium pH and feeding of the substrates glucose and phenylpyruvic acid (PPA)
on the production of phenyllactic acid (PLA) by Lactobacillus paracasei W2 in a 7.5 L fermentor were studied. The results
indicated that all three factors generated corresponding effects on cell growth and PLA production. Two substrate feeding
strategies, intermittent feeding of PPA and continuous feeding of both glucose and PPA were performed and the initial
medium pH was set to 6.5. After 36 h of fermentation under these conditions, PLA production reached 1.148 g/L and 2.121 g/L,
respectively, and the conversion rate of PPA reached 62.19% and 47.2%, respectively. In addition, in comparison with fedbatch
fermentation, the PLA yield was enhanced by 41.72% and 161.53%, respectively.

Key words: Lactobacillus paracasei, phenyllactic acid, phenylpyruvic acid, substrate feeding

CLC Number:

TQ921.3

WANG Li-mei1,2, TENG Yu1, CHEN Wen-fei1, ZHANG Hui1, CHU Kai-yang1. Effects of pH and Substrate Feeding on Phenyllactic Acid Production by Lactobacillus paracasei W2[J]. FOOD SCIENCE, 2014, 35(1): 163-166.

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