Abstract:

Purpose: To investigate the effect of resveratrol (Res) on proliferation of 3T3-L1 preadipocyte and its potential
molecular mechanisms. Methods: 3T3-L1 preadipocytes were plated in plastic dishes and treated with or without indicated
levels of Res (25, 50, 75, and 100 μmol/L). Cell toxicity of different doses of Res was assayed by MTT test. The mRNA
expression of LYRM1 was determined by real time polymerase chain reaction (RT-PCR), and the expressions of proapoptotic
protein Bak and anti-apoptotic protein Bcl-2 were confirmed by Western blot. Results: Res treatments dosedependently
suppressed the proliferation potential of preadipocyte, via reduced expression of LYRM1 mRNA, increased
Bak protein expression and decreased Bal-2 protein expression. Conclusion: Res treatments suppressed 3T3-L1 preadipocyte
proliferation, which may be associated with reduced LYRM1 expression and then mitochondrial dysfunction, finally leading
to progressive apoptotic process.

Key words: resveratrol, 3T3-L1 preadipocyte, proliferation, LYRM1, Bcl-2 family