Abstract:

To investigate the immunoregulatory effect of extracellular polysaccharides produced by Pleurotus ferulae Lanzi
immune in tumor-bearing mice, tumor-bearing mice were developed by subcutaneous injection of murine sarcoma S180
and intragastric administration. The numbers of leukocytes and lymphocytes in blood, peritoneal macrophage phagocytic
activities, splenic lymphocyte proliferation, immune organ weight indexes and tumor necrosis factor-α (TNF-α) level in
blood plasma were examined with an automatic blood analyzer, cell culture in vitro and enzyme-linked immunosorbent
assay, respectively. The results showed that extracellular polysaccharides produced by Pleurotus ferulae Lanzi at the
concentrations of 100 and 200 mg/kg caused a significant reduction in the weight of tumor (P ＜ 0.05) and a significant
increase in the numbers of leukocytes and lymphocytes in blood (P ＜ 0.05), the weight indexes of spleen and thymus gland
(P ＜ 0.05) and the concentration of TNF-α in blood plasma (P ＜ 0.05), and also significantly enhanced the proliferation capacity
of splenic lymphocytes (P ＜ 0.05); while phagocytic activities of murine peritoneal macrophages were significantly enhanced by
the extracellular polysaccharides at the concentration of 200 mg/kg (P ＜ 0.05). Thus, the immune function of tumor-bearing mice
could be improved by extracellular polysaccharides produced by Pleurotus ferulae Lanzi at a certain concentration.

Key words: Pleurotus ferulae Lanzi, extracellular polysaccharides, immune organ indexes, tumor necrosis factor-α, splenic lymphocytes