Isolation, Purification and Monosaccharide Analysis of Polysaccharide from Sipunculus nudus

doi:10.7506/spkx1002-6630-201501021

Abstract

Abstract:

Crude polysaccharide was obtained from Sipunculus nudus by alkali extraction and deproteinized by Sevag
method. The polysaccharide was sequentially purified by ion exchange chromatography on DEAE-52 cellulose column
and Sephadex G-100 Gel column. Then, the purified polysaccharide from Sipunculus nudus (SNPS) was characterized
for physicochemical properties and monosaccharide composition. The results showed that SNPS with a purity of 99.35%
contained 22.21% alduronic acid without protein, nuclein or sulfate ions. Gas chromatography analysis showed that the
monosaccharide compositions of SNPS were arabinose, glucose and galactose, with a molar ratio of 1.16:9.54:1. This study
demonstrated that SNPS was a homogeneous component obtained from Sipunculus nudus.

Key words: Sipunculus nudus, polysaccharide, cellulose, gel, monosacharide

CLC Number:

R284.2

DONG Lanfang, ZHANG Qin*, XU Mingzhu. Isolation, Purification and Monosaccharide Analysis of Polysaccharide from Sipunculus nudus[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201501021.

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Isolation, Purification and Monosaccharide Analysis of Polysaccharide from Sipunculus nudus

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