Isolation and Biological Characteristics of Lytic Salmonella Bacteriophage

doi:10.7506/spkx1002-6630-201505025

Abstract

Abstract:

Two lytic Salmonella bacteriophages, namely vB_SenM-PA13076 (PA13076) and vB_SenM-PC2184 (PC2184)
that can inactivate ATCC13076 or CVCC2184 were isolated from the sewage samples of poultry slaughtering plants. Their
biological properties were identified by plaque, host range (antibiotic-resistant bacteria), transmission electron microscopy
(TEM), genetic material, pH and thermostability, optimal multiplicity of infection (MOI) and one-step growth experiment.
Their plaques on host strains were clear and bright, and also caused bacteriolysis to other strains of Salmonella. PA13076
could strongly cause bacteriolysis to an ampicillin-resistant strain SE A+13076, while PC2184 only lyse another strain
A+2184 slightly. The newly isolated bacteriophages were the members of family Myoviridae according to their ultrastructure
under TEM. Their genomes were dsDNA and revealed excellent tolerance to pH and temperature. The MOI of PA13076
(PC2184) was 0.01 (10), and the latent period was 20 min (30 min), the burst period was 70 min (60 min) and the average
burst size was 21 (23) phages per cell.

Key words: Salmonella enteritidis, lytic phage, biological characteristic, multi-antibiotic resistance

CLC Number:

Q939.48

BAO Hongduo1, ZHANG Pengyu1,2, ZHOU Yan1, ZHANG Lili1, ZHANG Hui1, TAO Mingxuan2, WANG Ran1,*. Isolation and Biological Characteristics of Lytic Salmonella Bacteriophage[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201505025.

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Isolation and Biological Characteristics of Lytic Salmonella Bacteriophage

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