Abstract:

In order to obtain the optimal enzymatic extraction conditions for phycoerythrin from laver with both cellulase
and pectinase, the mixing ratio between the two enzymes was selected, and response surface methodology was used to
explore the effects of extraction temperature, extraction time, total enzyme amount, and pH as well as their interactions on
the yield and purity of phycoerythrin. The optimal extraction conditions were determined as follows: a cellulase to pectinase
ratio of 7:3 (m/m); pH 6.8, 39 ℃, 7.2 h, and a total enzyme amount of 0.04 g. Under these conditions, the extraction rate of
phycoerythrin was 2.257%, and the purity of phycoerythrin was 1.656. The predicted parameters obtained from this work
were accurate, and could be applied to extraction and production of phycoerythrin from lavers.

Key words: laver, phycoerythrin, extraction, mixed enzymes, response surface methodology