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Purification and Hypoglycemic Effect of Extracellular Polysaccharide from Pleurtus spodotecus Fr.

LI Can1,2, HU Fang1, ZHU Ruijuan1, CHEN Tongqiang2, DENG Xibei3, WANG Shumin3, FENG Shilan3,*   

  1. 1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China; 2. Hunan Institute of Food Quality Supervision Inspection and Research,
    Changsha 410111, China; 3. School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China研究长柄侧耳胞外多糖分离纯化方法及对糖尿病小鼠的降血糖作用。从长柄侧耳液体发酵液中提取出长
    柄侧耳胞外多糖，采用Sevag法除蛋白、H2O2脱色素、透析和Sepadex G-200、100柱色谱分离纯化得到一种分子质
    量单一的多糖组分PSEPS2-A，以凝胶渗透色谱与光散射仪联用技术（gel permeation chromatography-eighteen angle
    laser scattering detector，GPC-MALLS）和元素分析等其他化学分析法考察多糖的理化性质。以四氧嘧啶诱导建
    立糖尿病小鼠模型，通过不同剂量PSEPS2-A治疗，比较治疗前后小鼠体质量及空腹血糖值、糖耐量水平、脏器
    指数、肝组织肝糖原水平变化。结果表明：PSEPS2-A的分子质量为6.268×105 D，在0.9% NaCl溶液中为棒状结构的
    多糖。PSEPS2-A能显著降低糖尿病小鼠空腹血糖值（P＜0.05）和血糖曲线下面积（P＜0.05），减缓糖尿病小鼠体
    质量的负增长；显著降低肝脏、肾脏脏器指数（P＜0.05），显著升高肝糖原水平（P＜0.05），促进肝糖原合成。
    PSEPS2-A是一种新的长柄侧耳胞外多糖，在一定剂量下具有调节糖代谢、降低血糖、改善糖尿病症状的作用。
  • Online:2015-04-15 Published:2015-05-05

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Abstract:

This study was conceived to investigate the isolation, purification and hypoglycemic effect of extracellular
polysaccharides from Pleurtus spodotecus Fr. in diabetic mice. A homogeneous extracellular polysaccharide named as
PSEPS2-A was extracted from the fermented broth of Pleurtus spodotecus Fr.. Then PSEPS2-A was treated with Sevag
method, H2O2 and purified with Sephadex gel filtration chromatography. GPC-MALLS and other methods were utilized to
examine the physical and chemical properties of PSEPS2-A. The absolute molecular weight of PSEPS2-A was 6.268 × 105 D
and the structure was rod-like in 0.9% NaCl solution. Diabetic mice induced by Alloxan were subjected to continuous
intragastric administration with different doses of PSEPS2-A. The changes in body weight, fasting blood glucose (FBG),
glucose tolerance, organ index, and liver glycogen in diabetic mice were determined before and after the treatment.
PSEPS2-A could significantly reduce the content of FBG (P < 0.05) and the area under curve of blood glucose (P < 0.05).
The symptom of negative growth of body weights was slowed down and the organ index was decreased in diabetic mice.
PSEPS2-A also could significantly promote liver glycogen synthesis and elevate the level of liver glycogen. PSEPS2-A
is a new extracellular polysaccharide from Pleurtus spodotecus Fr., which plays an important role in regulating glucose
metabolism, lowering blood sugar, and improving diabetic symptoms at a certain concentration.

Key words: Pleurtus spodotecus Fr., extracellular polysaccharides, isolation and purification, diabetes, hypoglycemic

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