Abstract:

Totally 49 strains suspected to be lactic acid bacteria were segregated from pickled mantis shrimp. Three of
these strains, namely L14, L22 and L28, were preliminarily screened via morphological observation and physiological and
biochemical tests. Subsequent 16S rDNA sequencing and analysis showed that L14 had 99% similarity to Enterococcus
faecium, L22 had 98% similarity to Lactobacillus brevis and L28 had 99% similarity to Lactobacillus coryniformis subsp.
torquens. The values of optical density at 600 nm (OD600 nm) of the three selected strains after 24 h of culture were all higher
than 1.0 and the pH values of their cultures were around 4.0 with the lowest pH of 3.97 observed for L22. All these three
strains grew well at temperatures ranging from 25 to 35 ℃ and grew normally in the presence of 60 g/L NaCl.

Key words: pickled mantis shrimp, screening, lactic acid bacteria, 16S rDNA