Abstract:

Objective: To understand the relative contents and changes of Bacteroides, Bifidobacterium, Clostridium leptum
and Lactobacillus in the intestinal flora of healthy people and diabetic patients with and without genetic inheritance, drug
treatment or obesity for future prevention and treatment of diabetes. Methods: Fecal samples were collected from the above
populations (n = 10). The total DNAs of microorganisms were extracted from the samples, and specific gene fragments
from the four intestinal bacteria were amplified by fluorescence quantitative PCR. Differences and changes in these four
bacterial species were studied. Results: The relative contents of Bacteroides and Bifidobacterium in healthy population
were lower than those in diabetic population, while the relative contents of Clostridium leptum and Lactobacillus in healthy
population were higher than those in diabetic population. Among the diabetic population, the contents of Bacteroides and
Bifidobacterium after drug treatment were lower than those before drug treatment, while the relative contents of Clostridium
leptum and Lactobacillus were higher in patients without drug treatment. The contents of Bacteroides and Bifidobacterium
in obese patients with high blood glucose were lower than those of the patients with high blood glucose alone, while the
relative contents of Clostridium leptum and Lactobacillus were higher in the formers. Genetic inheritance had no statistical
significance. Conclusion: These four gut bacteria are closely related to the origination and development of diabetes. Their
contents in diabetic patients with drug treatment are close to those in healthy population. Obesity aggravates the development
of diabetes. Genetic inheritance has no effect on gut flora.

Key words: diabetes, gut flora, quantitative analysis, Bacteroides, Bifidobacterium, Clostridium leptum, Lactobacillus