Abstract:

The objective of the study was to extract high quality RNA from wax apple (Syzygium samarangense Merr. et
Perry) fruits. The effectiveness of five RNA extraction methods, i.e., Plant RNA Kit, hexadecyltrimethylammonium bromide
(CTAB), modified CTAB, sodium dodecyl sulfate (SDS) and Trizol, was evaluated. The results showed that the quality of
total RNA extracted by SDS method was poor due to serious RNA degradation and genomic DNA contamination. The total
RNA extracted with Trizol reagent contained various residues such as proteins and polysaccharides and exhibited no RNA
bands. Plant Kit protocol, CTAB and modified CTAB methods were found to be suitable for total RNA extraction from wax
apple fruit. RT-PCR analysis demonstrated that the RNA bands were consistent with the target fragment size, which showed
that the extracted total RNA could be applied to subsequent molecular biology research.

Key words: wax apple fruit, RNA isolation, polysaccharide, polyphenol, method