Abstract:

This paper reports the optimization of medium components and fermentation conditions for improved production
of prodigiosin by Serratia marcescen using single factor and orthogonal array designs. Results indicated that the optimal
medium consisted of 4 mL/100 g of soybean oil, 1.5% of peptone, 0.15% of sodium chloride and 0.15% of potassium
dihydrogen phosphate, and the optimal fermentation conditions were determined as 30 ℃, an inoculum size of 1%, 70 mL
of medium contained in a 250-mL flask, and cultivation with shaking at 200 r/min. The maximum prodigiosin production
achieved after 36 h of cultivation under the optimized conditions was 2.98 g/L, and the activity of extracellular lipase from
S. marcescens was 15 U/mL.

Key words: Serratia marcescens, prodigiosin, single factor method, orthogonal array design