Abstract:

In order to investigate the modulation of casein glycomacropeptide (CGMP) on the diversity of intestinal flora
in mice with ulcerative colitis (UC), an oxazolone-induced mouse model with ulcerative colitis was used to explore the
intestinal flora in the presence of CGMP and the mechanism underlying the treatment of ulcerative colitis through CGMP.
The mice with oxazolone-induced ulcerative colitis were divided into four groups including normal control group, model
control group, CGMP group and salazosulfapyridine (SASP) group. After oxazolone challenge, the mouse in model
control group were given regular feed as same as that provided to normal control group, and the mice in CGMP group
and SASP group were administrated with CGMP at 50 mg/(kg·d) and SASP at 40 mg/(kg·d) for 7 consecutive days. Ion
Torrent sequencing technology was applied to detect the diversity of intestinal flora in mice. Marked structural changes
were discovered in the gut microbiota of mice with ulcerative colitis, showing a reduction in intestinal flora diversity and
decline in dominant bacterial populations. The sequencing data were analyzed by multivariate statistical methods and
the results showed that the abundance of Bacteroidetes and Firmicutes were significantly decreased, whereas Actinobacteria
and Proteobacteria were increased. The intestinal bacterial diversity in UC mice was increased after the intervention of CGMP,
indicating that CGMP could improve the oxazolone-induced ulcerative colitis of mice by adjusting the imbalanced intestinal flora.

Key words: casein glycomacropeptide (CGMP), oxazolone, ulcerative colitis, intestinal microbiota, Ion Torrent PGM platform