Optimization of D-Phenyllactic Acid Production by Whole Cells of Recombinant Escherichia coli Using Response Surface Methodology

doi:10.7506/spkx1002-6630-201607017

Abstract

Abstract:

The bioconversion conditions for the production of D-3-phenyllactic acid from phenylpyruvic acid (PPA) using
whole cells of Escherichia coli BL21(DE3)/pET-28a-ldhD were studied. Using Plackett-Burman design, buffer pH, substrate
concentration and temperature were selected as the factors with significant influence on substrate conversion efficiency out
of 6 factors. Subsequently, the optimization of the three factors was carried out using Box-Behnken design and response
surface analysis. Results indicated that the optimal bioconversion conditions that provided the maximum molar conversion
efficiency of PPA (65.32%) during 30 min were buffer pH 7.0, substrate concentration 42 mmol/L, and temperature 38 ℃,
cell concentration 20 g/L and glucose concentration 20 g/L. Under these conditions, 119 mmol/L (19.75 g/L) of D-PLA with
a productivity of 4.94 g/(L·h) was obtained after 4 h transformation with intermittent PPA feeding.

Key words: D-phenyllactic acid, phenylpyruvic acid, recombinant Escherichia coli, biotransformation, response surface optimization

CLC Number:

TS201.2

WANG Ying, HE He, HU Fagen, QI Bin, WANG Limei, ZHU Yibo. Optimization of D-Phenyllactic Acid Production by Whole Cells of Recombinant Escherichia coli Using Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201607017.

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Optimization of D-Phenyllactic Acid Production by Whole Cells of Recombinant Escherichia coli Using Response Surface Methodology

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