Identification of Core Region of DNA Aptamers against Tetrodotoxin

doi:10.7506/spkx1002-6630-201704042

Abstract

Abstract: The secondary structures of DNA aptamers A3 and G10 against tetrodotoxin were analyzed using DNA folding form online software. Further, digoxin labeled 5’ end sequences of four regions in each aptamer were separately synthesized. Tetrodotoxin was fixed by acrylic acid-epoxy ethane beads. The core regions of these aptamers were identified using digoxin-anti digoxin alkaline phosphatase system to detect the affinity of these various regions with tetrodotoxin, after determining the appropriate dilution ratio of anti-digoxin alkaline phosphatase. The results showed that the suitable working concentration of anti-digoxin alkaline phosphatase was 2 × 104 times dilution. The core region of aptamer A3 was A3-2 area which had the strongest affinity with tetrodotoxin among the four regions, including 47 nucleotides, while the core region of aptamer G10 was G10-4 area, including 56 nucleotides.

Key words: tetrodotoxin, aptamer, core region, affinity

CLC Number:

SHAO Biying, CHEN Wenbing, LIU Zhengcai, MIAO Tingyu, PENG Juan, YANG Fang. Identification of Core Region of DNA Aptamers against Tetrodotoxin[J]. FOOD SCIENCE, 2017, 38(4): 260-264.

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Identification of Core Region of DNA Aptamers against Tetrodotoxin

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