Adhesion characteristics and carbohydrate metabolism pathway analysis of three Lacticaseibacillus paracasei strains from different sources based on genome sequencing analysis

Abstract

Abstract: Lacticaseibacillus paracasei ZY-1was derived from Tibetan kefir grains, L. paracasei S-NA5 and L. paracasei S-NB was isolated from Xinjiang traditional fermented milk. In this study, the surface characteristics and adhesion properties of the three strains were evaluated in vitro, while the difference of adhesion-related genes was analyzed and compared based on the whole genome sequencing. The gastrointestinal tolerance and adhesion properties of L. paracasei S-NB were superior to S-NA5 and ZY-1. Moreover, two exopolysaccharide (EPS) synthesis gene clusters were found in all of the three strains, while the EPS cluster 1 of strain ZY-1 differed significantly from those of S-NA5 and S-NB. Meanwhile, genes involved in lipoteichoic acid (LTA) synthesis and related domains of protein adhesins were predicted. The secondary structure of 11 adhesive proteins was dominated by α helix and random curling, according to the results of the secondary structure prediction analysis of the adhesive protein gene coding products from strain S-NB. Additionally, complete metabolic pathways of lactose, galactose, fructi-oligosaccharide, inulin and human milk oligosaccharides were found in three strains, which were highly conserved.

Key words: Lacticaseibacillus paracasei, intestinal adhesion, genome sequencing, adhesion factors, carbohydrate metabolism pathway

CLC Number:

TS201.3

Luyao Xiao Wei Li. Adhesion characteristics and carbohydrate metabolism pathway analysis of three Lacticaseibacillus paracasei strains from different sources based on genome sequencing analysis[J]. FOOD SCIENCE, 0, (): 0-0.

References

[1] CUI Yanhua, QU Xiaojun. Genetic mechanisms of prebiotic carbohydrate metabolism in lactic acid bacteria: Emphasis on Lacticaseibacillus casei and Lacticaseibacillus paracasei as flexible, diverse and outstanding prebiotic carbohydrate starters[J]. Trends in Food Science & Technology, 2021, 115: 486-499. DOI: 10.1016/j.tifs.2021.06.058.
[2] SUN Erna, ZHANG Xiaomei, ZHAO Yifan, et al. Beverages containing Lactobacillus paracasei LC-37 improved functional dyspepsia through regulation of the intestinal microbiota and their metabolites[J]. Journal of Dairy Science, 2021, 104(6): 6389-6398. DOI: 10.3168/jds.2020-19882.
[3] LV Xu-cong, CHEN Min, HUANG Zi-rui, et al. Potential mechanisms underlying the ameliorative effect of Lactobacillus paracasei FZU103 on the lipid metabolism in hyperlipidemic mice fed a high-fat diet[J]. Food Research International, 2021, 139: 109956. DOI: 10.1016/j.foodres.2020.109956.
[4] 占萌, 李春, 李慧臻, 等. 乳酸菌粘附肠道上皮细胞的研究进展[J]. 中国食品学报, 2020, 20(11): 341-350. DOI: 10.16429/j.1009-7848.2020.11.038.
[5] RUIZ Lorena, HEVIA Arancha, BERNARDO David, et al. Extracellular molecular effectors mediating probiotic attributes[J]. FEMS Microbiology Letters, 2014, 359(1): 1-11. DOI: 10.1111/1574-6968.12576
[6] CLAES IJJ, LEBEER Sarah, SHEN C, et al. Impact of lipoteichoic acid modification on the performance of the probiotic Lactobacillus rhamnosus GG in experimental colitis[J]. Clinical & Experimental Immunology, 2010, 162(2): 306-314. DOI: 10.1111/j.1365-2249.2010.04228.x.
[7] DU Yang, LI Hao, XU Wenlong, et al. Cell surface-associated protein elongation factor Tu interacts with fibronectin mediating the adhesion of Lactobacillus plantarum HC-2 to Penaeus vannamei intestinal epithelium and inhibiting the apoptosis induced by LPS and pathogen in Caco-2 cells[J]. International Journal of Biological Macromolecules, 2023, 224: 32-47. DOI: 10.1016/j.ijbiomac.2022.11.252.
[8] LU Yanmeng, HAN Shengyi, ZHANG Shuobo, et al. The role of probiotic exopolysaccharides in adhesion to mucin in different gastrointestinal conditions[J]. Current Research in Food Science, 2022, 5: 581-589. DOI: 10.1016/j.crfs.2022.02.015.
[9] XIAO Luyao, YANG Yao, HAN Shuo, et al. Effects of genes required for exopolysaccharides biosynthesis in Lacticaseibacillus paracasei S-NB on cell surface characteristics and probiotic properties[J]. International Journal of Biological Macromolecules, 2023, 224: 292-305. DOI: 10.1016/j.ijbiomac.2022.10.124.
[10] WU Jinsong, HAN Xiangpeng, YE Meizhi, et al. Exopolysaccharides synthesized by lactic acid bacteria: Biosynthesis pathway, structure-function relationship, structural modification and applicability[J]. Critical Reviews in Food Science and Nutrition, 2022, 1-22. DOI: 10.1080/10408398.2022.2043822.
[11] MENDE Susann, ROHM Harald, JAROS Doris. Influence of exopolysaccharides on the structure, texture, stability and sensory properties of yoghurt and related products[J]. International Dairy Journal, 2016, 52: 57-71. DOI: 10.1016/j.idairyj.2015.08.002.
[12] Rehm Bernd. Bacterial polymers: biosynthesis, modifications and applications[J]. Nature Reviews Microbiology, 2010, 8(8): 578-592. DOI: 10.1038/nrmicro2354.
[13] WHITFIELD Chris, WEAR Samantha, SANDE Caitlin. Assembly of bacterial capsular polysaccharides and exopolysaccharides[J]. Annual Review of Microbiology, 2020, 74: 521-543. DOI: 10.1146/annurev-micro-011420-075607.
[14] BRON Peter, van BAARLEN Peter, KLEEREBEZEM Michiel. Emerging molecular insights into the interaction between probiotics and the host intestinal mucosa[J]. Nature Reviews Microbiology, 2012, 10(1): 66-78. DOI: 10.1038/nrmicro2690.
[15] NILSEN Nadra, DEININGER Susanne, NONSTAD Unni, et al. Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36[J]. Journal of leukocyte biology, 2008, 84(1): 280-291. DOI: 10.1189/jlb.0907656
[16] GRANGETTE Corinne, NUTTEN Sophie, PALUMBO Emmanuelle, et al. Enhanced antiinflammatory capacity of a Lactobacillus plantarum mutant synthesizing modified teichoic acids[J]. Proceedings of the National Academy of Sciences, 2005, 102(29): 10321-10326. DOI: 10.1073/pnas.0504084102.
[17] MATSUGUCHI Tetsuya, TAKAGI Akimitsu, MATSUZAKI Takeshi, et al. Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through Toll-like receptor 2[J]. Clinical and Vaccine Immunology, 2003, 10(2): 259-266. DOI: 10.1128/CDLI.10.2.259-266.2003.
[18] MOHAMADZADEH Mansour, PFEILER Erika, BROWN Jeffrey, et al. Regulation of induced colonic inflammation by Lactobacillus acidophilus deficient in lipoteichoic acid[J]. Proceedings of the National Academy of Sciences, 2011, 108(supplement_1): 4623-4630. DOI: 10.1073/pnas.100506610.
[19] DESVAUX Micka?l, DUMAS Emilie, CHAFSEY Ingrid, et al. Protein cell surface display in Gram-positive bacteria: from single protein to macromolecular protein structure[J]. FEMS microbiology letters, 2006, 256(1): 1-15. DOI: 10.1111/j.1574-6968.2006.00122.x.
[20] BUCK B Logan, ALTERMANN Eric, SVINGERUD Tina, et al. Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM[J]. Applied and Environmental Microbiology, 2005, 71(12): 8344-8351.DOI: 10.1128/AEM.71.12.8344-8351.20
[21] HYMES Jeffery, KLAENHAMMER Todd. Stuck in the middle: Fibronectin-binding proteins in gram-positive bacteria[J]. Frontiers in Microbiology, 2016, 7: 1504. DOI: 10.3389/fmicb.2016.01504.
[22] CHAGNOT Caroline, LISTRAT Anne, ASTRUC Thierry, et al. Bacterial adhesion to animal tissues: protein determinants for recognition of extracellular matrix components[J]. Cellular microbiology, 2012, 14(11): 1687-1696. DOI: 10.1111/cmi.12002.
[23] SINGH B, FLEURY C, JALALVAND F, et al. Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host[J]. FEMS Microbiology Reviews, 2012, 36(6): 1122-1180. DOI: 10.1111/j.1574-6976.2012.00340.x.
[24] YADAV Ashok Kumar, TYAGI Ashish, KAUSHIK Jai Kumar, et al. Role of surface layer collagen binding protein from indigenous Lactobacillus plantarum 91 in adhesion and its anti-adhesion potential against gut pathogen[J]. Microbiological Research, 2013, 168(10): 639-645. DOI: 10.1016/j.micres.2013.05.003.
[25] LEHRI B, SEDDON AM, KARLYSHEV AV. Lactobacillus fermentum 3872 as a potential tool for combatting Campylobacter jejuni infections[J]. Virulence, 2017, 8(8): 1753-1760. DOI: 10.1080/21505594.2017.1362533.
[26] HYMES JP, JOHNSON BR, BARRANGOU R, et al. Functional analysis of an S-layer-associated fibronectin-binding protein in Lactobacillus acidophilus NCFM[J]. Applied and Environmental Microbiology, 2016, 82(9): 2676-2685. DOI: 10.1128/AEM.00024-16.
[27] ISKANDAR Christelle F, CAILLIEZ-GRIMAL Catherine, BORGES Frédéric, et al. Review of lactose and galactose metabolism in Lactic Acid Bacteria dedicated to expert genomic annotation[J]. Trends in Food Science & Technology, 2019, 88: 121-132. DOI: 10.1016/j.tifs.2019.03.020.
[28] BARRANGOU Rodolphe, ALTERMANN Eric, HUTKINS Robert, et al. Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus[J]. Proceedings of the National Academy of Sciences, 2003, 100(15): 8957-8962. DOI: 10.1073/pnas.1332765100.
[29] SAULNIER Delphine M A, MOLENAAR Douwe, de VOS Willem M, et al. Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays[J]. Applied and Environmental Microbiology, 2007, 73(6): 1753-1765. DOI: 10.1128/AEM.01151-06.
[30] SMILOWITZ Jennifer T, LEBRILLA Carlito B, MILLS David A, et al. Breast milk oligosaccharides: structure-function relationships in the neonate[J]. Annual Review of Nutrition, 2014, 34: 143-169. DOI: 10.1146/annurev-nutr-071813-105721.