Abstract: In order to explore the effect of basigin (BSG) on MAPK signaling pathway and apoptosis during post-slaughter maturation of Qinchuan cattle, the changes of BSG and its differential proteins were analyzed by 4D-label free quantification (4D-LFQ). The BSG inhibitor thinomycin was injected into the longest dorsal muscle of Qinchuan beef after slaughter, and the expression level of key proteins of MAPK pathway during storage at 4°C was determined by western blotting. The apoptosis detection kit was used to determine the viability change of caspase-3. In this way, the effects of BSG inhibitors on MAPK signaling pathway and apoptosis were analyzed. The results showed that the expression of BSG increased first and then decreased during the storage period of Qinchuan cattle. KEGG pathway analysis was used to show that BSG and its differential proteins were significantly annotated in oxidative phosphorylation pathway, calcium signaling pathway and MAPK signaling pathway, indicating that BSG played a role through MAPK pathway. In addition, the relative expression of key proteins of the MAPK pathway in the thinomycin group was significantly down-regulated, indicating that BSG inhibitors inactivated the MAPK signaling pathway. This lays a good foundation for studying the effect of BSG on the MAPK signaling pathway. After inhibiting the expression of BSG, the activity of caspase-3 in the hiromycin group increased significantly, indicating that apoptosis was an important link in cell damage, and thinomycin acted on the N-terminal structure of BSG to deglycosylation of proteins. By inhibiting the folding of proteins within cells, their biological activity is inhibited, thereby inducing apoptosis in cells.

Key words: Qinchuan bovine dorsal longest muscle, MAPK signaling pathway, Thinomycin, apoptosis, Cell damage