Preparation of Colloidal Gold Strip for the Detection of Escherichia coli O157:H7

doi:10.7506/spkx1002-6630-201024078

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 355-359.doi: 10.7506/spkx1002-6630-201024078

• Processing Technology • Previous Articles Next Articles

Preparation of Colloidal Gold Strip for the Detection of Escherichia coli O157:H7

HUANG Ling-fang1，DUAN Xia1，CHEN Yuan2，LIU Wen-juan2，WEI Hua1，LAI Wei-hua1,*

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China ；
2. Jiangxi Zodolabs Biological Engineering Co. Ltd., Nanchang 330029, Chin

Received:2010-08-06 Revised:2010-11-14 Online:2010-12-25 Published:2010-12-29
Contact: LAI Wei-hua1 E-mail:talktolaiwh@163.com

Abstract

Abstract:

Anti-Escherichia coli O157:H7 monoclonal antibody was labeled with colloidal gold to prepare a strip for the detection of E. coli O157: H7. Anti-E. coli O157:H7 polyclonal antibody and donkey anti-mouse IgG were dispensed on the nitrocellulose membrane as the test line and control line, respectively. The optimal conjugation was performed using 16.8μg/mL antibody at the condition of pH 8.0 and blocked with PEG20000. However, the optimal pH range for the detection was 7.0－7.5. Cross-reaction test was conducted by 26 bacteria strains. Results showed no obvious cross-reaction were observed except for the slight cross-reaction from Salmonella typhimurium ATCC13311 and Staphylococcus aureus CMCC26003. Meanwhile, the sensitivity of the developed strip was up to 104 CFU/mL. Moreover, this kind of developed strip was characteristics of simple operation, rapid detection, high sensitivity and specificity.

Key words: Escherichia coli O157:H7, colloidal gold, strip

CLC Number:

TS207.4

HUANG Ling-fang1，DUAN Xia1，CHEN Yuan2，LIU Wen-juan2，WEI Hua1，LAI Wei-hua1,*. Preparation of Colloidal Gold Strip for the Detection of Escherichia coli O157:H7[J]. FOOD SCIENCE, 2010, 31(24): 355-359.

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Preparation of Colloidal Gold Strip for the Detection of Escherichia coli O157:H7

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