食品科学 ›› 2011, Vol. 32 ›› Issue (19): 100-103.doi: 10.7506/spkx1002-6630-201119022

• 基础研究 • 上一篇    下一篇

花红可溶性膳食纤维抗氧化活性

牛广财,朱丹,肖盾,魏文毅,王鹤霖   

  1. 1. 黑龙江八一农垦大学食品学院
    2. 黑龙江八一农垦大学生命科学与技术学院
    3. 黑龙江九三油脂集团有限公司
  • 发布日期:2011-10-12
  • 基金资助:
    黑龙江省普通高等学校青年学术骨干支持计划项目(1154G59)

Antioxidant Activity of Soluble Dietary Fiber from Crab Apple Pomace

NIU Guang-cai1,ZHU Dan2,XIAO Dun3,WEI Wen-yi1,WANG He-lin1   

  1. (1. College of Food, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. Heilongjiang Jiusan Oil & Fat Co. Ltd., Harbin 150090, China)
  • Published:2011-10-12

摘要: 目的:以混合菌种发酵法制备的花红可溶性膳食纤维(MPSDF)为原料,研究其对 ·OH、O2- ·和DPPH自由基3种自由基的清除作用,以及对大豆油的抗氧化效果。方法:采用 ·OH、O2- ·体系、DPPH自由基体系和油脂过氧化值测定法,测定MPSDF对 ·OH、O2- ·和DPPH自由基的清除率以及Schaal烘箱法对大豆油过氧化值抑制效果。结果:MPSDF对 ·OH和O2- ·均有较强的清除能力,其EC50分别为4.63mg/mL和8.84mg/mL,但对DPPH自由基的清除率较低。MPSDF添加到大豆油中,其抗氧化效果与丁基羟基茴香醚(BHA)接近。结论:发酵法制备的花红可溶性膳食纤维(MPSDF)对 ·OH和O2- ·均有较强的清除作用,对大豆油有很强的抗氧化作用,具有较强的抗氧化活性。

关键词: 沙果, 可溶性膳食纤维, 清除自由基, 抗氧化

Abstract: Objective: To explore the antioxidant activity of soluble dietary fiber (SDF) prepared by fermenting crab apple (Malus asiatica) pomace with mixed strains. Methods: The scavenging rates of SDF derived from crab apple pomace against hydroxyl, superoxide anion and DPPH radicals and its inhibitory effect against lipid peroxidation in soybean oil were evaluated. Results: The SDF revealed strong scavenging capacity against hydroxyl and superoxide anion free radicals with an EC50 of respectively 4.63 mg/mL and 8.84 mg/mL, but the scavenging capacity against DPPH free radicals was weaker. The antioxidant effect of the SDF on soybean oil was similar to that of BHA. Conclusion: SDF derived from crab apple pomace has a strong scavenging capacity agaisnt hydroxyl, superoxide anion free radicals and an obvious antioxidant effect on soybean oil. Therefore, it can be applied as a food antioxidant, which will have promising application prospects.

Key words: crab apple, soluble dietary fiber, scavenging free radical, antioxidation

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