食品科学 ›› 2009, Vol. 30 ›› Issue (3): 184-187.doi: 10.7506/spkx1002-6630-200903041

• 生物工程 • 上一篇    下一篇

产L-乳酸的菌种的筛选及L-乳酸生产新途径

杨 萍,徐 忠   

  1. 哈尔滨商业大学食品工程学院
  • 收稿日期:2008-03-10 修回日期:2008-05-07 出版日期:2009-02-01 发布日期:2010-12-29
  • 通讯作者: 杨萍 E-mail:ping8434@163.com
  • 基金资助:

    国家自然科学基金项目(50278024)

Screening of Lactobacillus casei Fermenting Enzymatic Hydrolysate of Soybean Straw for Production of L-Lactic acid and Its Fermentation Characteristics

YANG Ping,XU Zhong   

  1. (College of Food Engineering, Harbin University of Commerce, Harbin 150076, China)
  • Received:2008-03-10 Revised:2008-05-07 Online:2009-02-01 Published:2010-12-29
  • Contact: YANG Ping, E-mail:ping8434@163.com

摘要:

本实验以大豆秸秆酶解液为原料,发酵制备L- 乳酸。首先对产L- 乳酸的四株菌进行筛选,选出性能优良、糖利用率和乳酸产量均较高的干酪乳杆菌作为实验用菌种。然后对乳酸菌发酵大豆秸秆酶解液制备L- 乳酸的影响因素进行了研究,研究结果表明:干酪乳杆菌发酵的最佳接种量为10%,最适发酵温度为30℃,最佳pH 值为5.5;在发酵42h 时,大豆秸秆酶解液中残留糖浓度接近于零,此时,随底物浓度的增高,乳酸产量相应增加,并且酶解液浓度达到34.98g/L 时,乳酸生产没有受到抑制。

关键词: 大豆秸秆, 乳酸菌, 发酵, L-乳酸.

Abstract:

This study aimed to produce L-lactic acid from enzymatic hydrolysis solution of soybean straw by fermentation. Lactobacillus casei was selected out from four species of Lactobacillus for producing L-lactic acid because of its good performance and high reducing sugar utilization rate and L-lactic acid yield. And its fermentation characteristics were investigated. The results indicated that the optimum temperature, inoculation size and pH are 30℃, 10% and 5.5, repectively; With sugar concentration rising, the lactic acid yield produced by Lactobacillus casei increases correspondingly. After 24-h fermentation, the residual concentration of sugar in hydrolysis solution of soybean straw is close to zero. At that time, the L-lactic acid yield increases with the substrate concentration increasing. When the substrate concentration is up to 34.98 g/L, the production of L-lactic acid is not inhibited any more.

Key words: soybean straw, Lactobacillus casei, fermentation, L-lactic acid

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