食品科学 ›› 2009, Vol. 30 ›› Issue (17 ): 244-246.doi: 10.7506/spkx1002-6630-200917056

• 生物工程 • 上一篇    下一篇

高浓度发酵及膜分离技术在2- 酮基-D- 葡萄糖酸生产中的应用研究

孙文敬1, 魏 转2, 林 琳1,余泗莲3,周 强3   

  1. 1.江苏大学食品与生物工程学院 2.河北化工医药职业技术学院制药工程系 3.百勤异VC 钠有限公司
  • 收稿日期:2009-05-19 出版日期:2009-09-01 发布日期:2014-04-14
  • 通讯作者: 孙文敬1, E-mail:sunwenjing1919@163.com
  • 基金资助:

    江苏大学科研启动基金项目(08JDG029);江西省主要学科学术和技术带头人培养计划项目(2008DD00600)

Application of High Concentration Fermentation and Membrane Separation Technology in the Production of 2-Keto-D-gluconic Acid

SUN Wen-jing1,WEI Zhuan2,LIN Lin1,YU Si-lian3,ZHOU Qiang3   

  1. 1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
    2. Department of Pharmaceutical, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China;
    3. Parchn Sodium Isovitamin C Co. Ltd., Dexing 334221, China
  • Received:2009-05-19 Online:2009-09-01 Published:2014-04-14
  • Contact: SUN Wen-jing1, E-mail:sunwenjing1919@163.com

摘要:

研究中和剂和底物总浓度对2- 酮基-D- 葡萄糖酸补料分批发酵的影响,并初步考察膜分离技术在发酵产物提取中应用的可行性。结果表明:在2- 酮基-D- 葡萄糖酸的补料分批发酵中,以NaOH 或Na2CO3 代替CaCO3 作为中和剂,能够有效提高发酵培养基中的底物总浓度,且发酵转化率可达90% 左右;在NaOH 或Na2CO3 作为发酵中和剂的情况下,膜技术可应用于2- 酮基-D- 葡萄糖酸的提取中,且有助于其提取率的提高。

关键词: 2- 酮基-D- 葡萄糖酸, 高浓度发酵, 膜分离技术, 提取

Abstract:

The effects of neutralization agents and total concentration of substrate on the fed-batch fermentation of 2-keto- D-gluconic acid were investigated and the feasibility of application of membrane separation technology to the extraction of fermentation product was explored. For the fed-batch fermentation of 2-keto-D-gluconic acid, to replace CaCO3 with NaOH or Na2CO3 as a neutralization agent, could significantly increased the total concentration of substrate in fermentation medium. In this case, the conversion rate of glucose into 2-keto-D-gluconic acid was as high as 90% and membrane technology could be applied to the separation of 2-keto-D-gluconic acid, facilitating the steady increase of overall production yield and the improvement of product quality.

Key words: 2-keto-D-gluconic acid, high concentration fermentation, membrane separation technology, extraction

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