食品科学 ›› 2008, Vol. 29 ›› Issue (3): 56-59.

• 基础研究 • 上一篇    下一篇

聚γ-谷氨酸延缓磷酸钙沉淀及螯合钙离子的研究

 夏芳, 蔡皓, 陈守文   

  1. 农业微生物学国家重点实验室 华中农业大学生命科学技术学院; 农业微生物学国家重点实验室; 华中农业大学生命科学技术学院; 湖北武汉430070;
  • 出版日期:2008-03-15 发布日期:2011-08-24

Effects of Poly-γ-glutamic Acid on Retarding Calcium Phosphate Depositing and It’s Chelation with Calcium Ion

 XIA  Fang, CAI  Hao, CHEN  Shou-Wen   

  1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2008-03-15 Published:2011-08-24

摘要: 研究表明聚γ-谷氨酸(γ-PGA)有延缓磷酸钙沉淀形成的能力,在γ-PGA添加量达到200mg/L的条件下,体系中减少磷酸钙沉淀形成率达83.4%,延缓达到最大沉淀量所需时间20min。同时还研究了γ-PGA与钙离子螯合形成螯合钙的条件,结果显示在温度37℃、pH6、3%γ-PGA、羧钙比2.5:1的条件下螯合率最高,螯合反应在15min完成;螯合物的钙含量为11.06%。γ-PGA能与钙离子形成可溶性钙,可开发成一种新型的补钙剂。

关键词: 聚&gamma, -谷氨酸, 钙离子, 延缓磷酸钙沉淀, 螯合率

Abstract: The ability of poly-γ-glutamic acid(γ-PGA)to retard calcium phosphate depositing was investigated in the study. When 200 mg/Lγ-PGA was added, it remarkably reduced the calcium phosphate depositing by 83.4%, and retarded the time of achieving the highest depositing to 20 min. The chelation conditions ofγ-PGA with calcium ion such as pH, temperature and the concentration of γ-PGA were studied. Results showed that the maximum yield of chelation was gained under the condition of temperature 37 ℃, pH 6.0, 3% γ-PGA and the molar ratio of carboxyl group ofγ-PGA to calcium ion 2.5:1, then the chelation course was finished within 15 min and the content of calcium was 11.06% in the chelate complex. It illustrated thatγ-PGA was able to chelate with calcium ion and worth exploiting as a new calcium supplemental agent.

Key words: poly-&gamma, -glutamic acid; calcium ion; retarding calcium phosphate depositing; yield of chelation;