食品科学 ›› 2008, Vol. 29 ›› Issue (5): 307-310.

• 生物工程 • 上一篇    下一篇

冬虫夏草与韩芝液体混合发酵条件研究

 武忠伟, 赵新亮, 窦艳萍, 赵现方   

  1. 河南科技学院生物工程系; 河南科技学院农学系; 河南科技学院生物工程系;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Study on Submerged Mixed Fermentation Condition of Cordyceps sinensis and Ganoderma lucidum HG

 WU  Zhong-Wei, ZHAO  Xin-Liang, DOU  Yan-Ping, ZHAO  Xian-Fang   

  1. 1.Department of Bioengineering,Henan Institute of Science and Technology;2.Department of Agriculture,Henan Institute of Science and Technology
  • Online:2008-05-15 Published:2011-08-26

摘要: 平板培养基上比较了冬虫夏草、韩芝及树舌的菌丝生长速度和对淀粉的利用情况,结果显示三者的生长速度分别达到1.28、0.64和0.55cm/d,且韩芝与树舌对淀粉的利用均优于冬虫夏草;混合培养结果得出菌种的最佳菌龄比为虫草:韩芝=3d:5d,最佳的接种比例为2:3;正交试验混合发酵最佳的培养基配方为(%):葡萄糖1.0、淀粉2.0、黄豆粉2.0、酵母粉0.5。在此条件下,菌丝生物量达到最大值1.97g/100ml。

关键词: 冬虫夏草, 韩芝, 混合培养

Abstract: The mycelium growth speed and the decomposition ability to starch of Cordyceps sinensis,Ganoderma lucidum HG and Ganoderma applanatumuan were compared on the flat medium.It was found that their mycelium growth speed reached 1.28 cm/d,0.64 cm/d,0.55 cm/d respectively,and the utilization ability to the starch Ganoderma lucidum and Ganoderma applanatumuan were superior to Cordyceps sinensis.The mixed culture results showed the optimum inoculation age of Cordyceps sinensis to Ganoderma lucidum HG was 3 d to 5 d,also the optimum inoculation proportion of Cordyceps sinensis to Ganoderma lucidum HG was 2:3;The best fermentation medium was tested by orthogonal design,and the the optimum nutrient ingredients combination(%):glucose 1.0,starch 2.0,soya flour 2.0,and yeast extract 0.5.on these conditions,the verification experimental results showed and the maximal biomass is 1.97 g/100 ml.

Key words: Cordyceps sinensis, Ganoderma lucidum HG, mixed culture