食品科学 ›› 2002, Vol. 23 ›› Issue (7): 68-70.

• 工艺技术 • 上一篇    下一篇

酶法提取可德兰多糖的研究

 李卫旗, 李志昂, 何国庆   

  1. 浙江大学生命科学学院,浙江大学农业工程与食品学院
  • 出版日期:2002-07-15 发布日期:2011-12-31

Study on Curdlan Extraction by Neutral Proteinase on Ferment Liquid

 LI  Wei-Qi, LI  Zhi-Ang, HE  Guo-Qing   

  • Online:2002-07-15 Published:2011-12-31

摘要: 经正交试验确定了用中性蛋白酶处理发酵液提取可德兰多糖的最优工艺条件。测定结果证明,该法较传统的多糖提取方式可更有效地去除菌体中的氨基氮、核酸和其它杂质类多糖,所获可德兰产品为纯一的β-(1,3)葡聚糖,并保持了正常的分子构型。

关键词: 可德兰, 蛋白酶, 提纯, 色谱

Abstract: As the result of orthogonal test,the most rational technologicl factors for extracting Curdlan fromferment liquid by means of the neutral proteinase was determined.It confirmed th at this extracting way was more effic ient than the other ways.The amino acids and nucleic acids of the bacteria were thoroughly removed in the product.The polysaccharine was identified to be theβ-(1,3)polyglucosan with proper molecular structure.

Key words: Curdlan , Proteinase , Extracting , Chro matograph