藠头蛋白质分离及抗肿瘤作用的研究

食品科学 ›› 2013, Vol. 34 ›› Issue (1): 300-302.

藠头蛋白质分离及抗肿瘤作用的研究

刘巍，丁学知*，夏立秋，孙运军，禹智辉，肖秀情，罗超枝，黄少亚

湖南师范大学生命科学学院，微生物分子生物学湖南省重点实验室

收稿日期:2011-10-19 修回日期:2012-12-06 出版日期:2013-01-15 发布日期:2013-01-07
通讯作者: 丁学知 E-mail:dingxuezhi@hunnu.edu.cn
基金资助:
湖南省科技计划项目;长沙市科技计划项目

Isolation and Antitumor Activity of Proteins from Allium chinense Bulbs

Received:2011-10-19 Revised:2012-12-06 Online:2013-01-15 Published:2013-01-07

摘要/Abstract

摘要： 利用Tris-HCl(pH7.5，100mmol/L)提取藠头总蛋白质，用不同饱和度硫酸铵盐析沉淀，检测饱和硫酸铵盐析后藠头蛋白质对小鼠B16黑色素瘤细胞和Meth-A细胞的抑制作用。结果表明：20%～80%饱和硫酸铵盐析制备的藠头蛋白质对白色念球菌、小鼠黑色素瘤细胞的生长均有明显的细胞毒效应，其中80%饱和硫酸铵盐析制备藠头总蛋白质抗菌抗肿瘤活性最为明显。藠头蛋白质经液相色谱-质谱(LC-MS)检测，其主要成分为凝集素、热休克蛋白、蒜氨酸酶、半胱氨酸合酶和肿瘤坏死因子受体蛋白等物质。

关键词: 藠头, 蛋白质, 黑色素瘤, 抗肿瘤作用, 液相色谱-质谱

Abstract: Allium chinense bulbs were extracted with pH 7.5 100 mmol/L Tris-HCl buffer solution and salted out with ammonium sulfate at different degrees of saturation to obtain total proteins. The antitumor activity of total protein extracts against mouse B16 melanoma cells and Meth-A cells was tested. Protein extracts obtained with 20%—80% saturated ammonium sulfate had potent cytotoxicity against both tumor cells and protein extract with the most potent cytotoxicity was obtained with 80% saturated ammonium sulfate, which mainly consisted of alliinase, heat shock protein, lectin, cysteine synthase and members of the tumor necrosis factor receptor super family as shown by LC-MS analysis.

Key words: Allium chinenese, protein, melanoma, anti-tumor, LC-MS

中图分类号:

刘?巍，丁学知*，夏立秋，孙运军，禹智辉，肖秀情，罗超枝，黄少亚. 藠头蛋白质分离及抗肿瘤作用的研究[J]. 食品科学, 2013, 34(1): 300-302.

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