食品科学

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新疆产大叶白麻不同部位黄酮类成分含量分析

蔡卿龄,张 勇,魏原杰,石秋梅*   

  1. 新疆大学生命科学与技术学院,新疆 乌鲁木齐 830046
  • 出版日期:2014-11-25 发布日期:2014-12-09
  • 基金资助:

    新疆维吾尔自治区自然科学基金项目(2012211A021)

Determination of Major Flavonoid Contents in Different Organs of Poacynum hendersonii (Hook.f.) Woodson Grown in Xinjiang

College of Life Science and Technology, Xinjiang University, Ürümqi 830046, China   

  1. College of Life Science and Technology, Xinjiang University, Ürümqi 830046, China
  • Online:2014-11-25 Published:2014-12-09

摘要:

采用高效液相色谱法测定新疆产大叶白麻不同部位金丝桃苷、异槲皮苷、芦丁及总槲皮素的含量;以NaAc-A1C13体系为显色剂,用分光光度法于波长420 nm处测定大叶白麻不同部位总黄酮的含量。结果表明:大叶白麻叶、花、根和茎中的总黄酮含量分别为1.39%、0.89%、0.30%和0.13%。在所检测的3 个主要黄酮类成分中,异槲皮苷含量最高,除根外,在叶、花和茎中均可检测,含量分别为0.4%、0.15%和0.07%;芦丁和金丝桃苷含量较低。采用高效液相色谱法检测叶片和花中酸水解后总槲皮素含量,结果表明,叶和花中槲皮素含量分别为0.63%和0.34%。

关键词: 大叶白麻, 黄酮类成分, 高效液相色谱

Abstract:

The contents of hyperoside, isoquercitrin, rutin and total quercetin in different parts of Poacynum hendersonii
(Hook.f.) Woodson from Xinjiang were determined by high performance liquid chromatography (HPLC), and the total
flavoniods content was determined by spectrophotometry at 420 nm. The results showed that the total flavoniod content was
1.39% in leaves, 0.89% in flowers, 0.30% in roots, and 0.13% in stems. Among three major flavonoids identified in this
plant, isoquercitrin was the most abundant and showed detectable levels (0.4%, 0.15% and 0.07% in leaves, flowers and
stems, respectively) in all three other parts except for roots, along with very small amounts of hyperoside and rutin. The total
quercetin content in leaves and flowers was 0.63% and 0.34% by HPLC following acidic hydrolysis, respectively.

Key words: Poacynum hendersonii (Hook.f.) Woodson, flavonoid contents, high performance liquid chromatography (HPLC)

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