食品科学

• 基础研究 • 上一篇    下一篇

蓝圆鲹蛋白酶解物的螯合矿物离子活性研究

杨伊然1,2,胡 晓1,杨贤庆1,*,李来好1,陈胜军1,吴燕燕1,林婉玲1,黄 卉1,马海霞1   

  1. 1.中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,国家水产品加工技术研发中心,广东 广州 510300;2.大连海洋大学食品科学与工程学院,辽宁 大连 116023
  • 出版日期:2017-02-15 发布日期:2017-02-28

Mineral Ion Chelating Activity of Enzymatic Protein Hydrolysates from Decapterus maruadsi Muscle

YANG Yiran1,2, HU Xiao1, YANG Xianqing1,*, LI Laihao1, CHEN Shengjun1, WU Yanyan1, LIN Wanling1, HUANG Hui1, MA Haixia1   

  1. 1. Key Laboratory of Aquatic Producut Processing, Ministry of Agriculture, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciencces, Guangzhou 510300, China; 2. College of Food Science and Engineering, Dalian Ocean University, Dalian 116023
  • Online:2017-02-15 Published:2017-02-28

摘要: 以蓝圆鲹为原料,采用胰蛋白酶、木瓜蛋白酶及碱性蛋白酶对其进行水解,研究酶解产物与钙、亚铁、锌离子的螯合活性。结果表明:蓝圆鲹经胰蛋白酶水解,水解度为23.14%时其产物具有较高螯合铁、锌离子的活性,其螯合率分别高达96.63%和94.28%;蓝圆鲹经木瓜蛋白酶水解,水解度为22.00%时,其产物具有较高螯合钙离子的活性,其螯合率高达96.78%;同时研究表明上述两种酶解物还具有较好的抗氧化活性,其1,1-二苯基-2-三硝基苯肼半抑制浓度值分别为3.74 mg/mL和3.64 mg/mL。此外,氨基酸分析表明蓝圆鲹高螯合矿物离子活性的酶解物中天冬氨酸、谷氨酸、赖氨酸含量较高。

关键词: 蓝圆鲹, 酶解物, 螯合活性, 矿物离子, 抗氧化

Abstract: In this research, we studied the chelating activity of Decapterus maruadsi muscle hydrolyzed by three different proteases, trypsin, papain and alcalase, towards Ca2+, Fe2+ and Zn2+. The results showed that the hydrolysates produced by trypsin (DH = 23.14%) had the highest chelating activity towards Fe2+ and Zn2+ with chelating rates of 96.63% and 94.28%, respectively. The hydrolysates by papain (DH = 22.0%) had the highest Ca2+ chelating activity with a chelating rate of 96.78%. It was also shown that both enzymatic hydrolysates had good antioxidant activity. The half maximal inhibitory concentration (IC50) values of the trypsin and papain hydrolysates for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were 3.74 and 3.64 mg/mL, respectively. In addition, the amino acid sequence analysis showed that the hydrolysates with higher contents of Glu, Asp and Lys had higher mineral ion chelating activity.

Key words: Decapterus maruadsi, hydrolysate, chelating activity, mineral ions, antioxidant

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