野山杏果肉总有机酸对高脂血症大鼠血脂及相关基因表达的影响

doi:10.7506/spkx1002-6630-201821026

食品科学 ›› 2018, Vol. 39 ›› Issue (21): 171-176.doi: 10.7506/spkx1002-6630-201821026

野山杏果肉总有机酸对高脂血症大鼠血脂及相关基因表达的影响

阿依姑丽·艾合麦提，王妙颖，彭禛菲，冯雅丽，王小燕，古扎丽努尔·艾尼

新疆农业大学食品科学与药学学院，新疆乌鲁木齐 830052

出版日期:2018-11-15 发布日期:2018-11-21
基金资助:
国家自然科学基金地区科学基金项目（31460393）

Effect of Total Organic Acid from Wild Apricot on Blood Lipid and the Expression of Related Genes in Hyperlipidemic Rats

Aygul·AHMAT, WANG Miaoying, PENG Zhenfei, FENG Yali, WANG Xiaoyan, Guzalnur·GENI

College of Food Science and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, China

Online:2018-11-15 Published:2018-11-21

摘要/Abstract

摘要： 目的：研究野山杏果肉总有机酸（total organic acids from wild apricot，TOAWA）对高脂血症大鼠的降血脂作用及机理。方法：建立高脂血症大鼠模型，以灌胃血脂康大鼠为阳性对照组，利用试剂盒测定血脂浓度和载脂蛋白质量浓度；利用实时荧光定量聚合酶链式反应（real-time polymerase chain reaction，qPCR）测定大鼠肝脏过氧化物酶体增殖物激活受体α（peroxisome proliferator-activated receptor-α，PPAR-α）和低密度脂蛋白受体（low-density lipoprotein receptor，LDL-R）的基因表达水平；制作实验大鼠的肝脏组织切片，观察其对肝脏组织结构的影响；探究TOAWA对高脂血症大鼠血脂的影响及作用机理。结果：TOAWA能极显著降低灌胃高脂乳剂实验大鼠的总胆固醇、甘油三酯和低密度脂蛋白胆固醇（low-density lipoprotein cholesterol，LDL-C）浓度（P＜0.01），显著降低载脂蛋白（apoprotein，Apo）B质量浓度（P＜0.05），极显著升高ApoA-I质量浓度和ApoA-I/Apo-B（P＜0.01），显著升高高密度脂蛋白胆固醇浓度（P＜0.05）。qPCR结果显示，灌胃高脂乳剂实验大鼠肝脏PPAR-α和LDL-R基因表达量降低，给予TOAWA干预后，实验大鼠肝脏PPAR-α和LDL-R基因表达量有明显的升高。肝脏组织切片的观察结果显示，TOAWA可明显改善肝功能。结论：TOAWA能调节高脂血症大鼠的血脂浓度，其作用与TOAWA调节Apo质量浓度、上调肝脏PPAR-α和LDL-R基因表达量及改善肝功能有关。

关键词: 野山杏果肉总有机酸, 高脂血症, 血脂, 作用机制

Abstract: Objective: To investigate the effect of total organic acid from wild apricot (TOAWA) on blood lipids and to explore the hypolipidemic mechanism of TOAWA in hyperlipidemic rats. Methods: A rat model of hyperlipidemia was established by orally feeding high-fat emulsion. Rats administered with Xuezhikang, a hypolipidemic agent were used as a positive control group. Commercial kits were employed to measure blood lipids and apolipoprotein. The mRNA expression levels of peroxisome proliferator-activated receptor-α (PPAR-α) and low-density lipoprotein receptor (LDL-R) in liver were measured by real-time polymerase chain reaction (qPCR), and the effect of TOAWA on blood lipids and pathological changes in liver was evaluated. Furthermore, the hypolipidemic mechanism of TOAWA was explored. Results: TOAWA could significantly decrease the levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C) (P < 0.01) and apoprotein B (Apo-B) (P < 0.05), and increase the levels of high-density lipoprotein cholesterol (HDL-C) (P < 0.05), apoprotein A-1 (ApoA-I) and ApoA-I/Apo-B ratio in the serum of hyperlipoidemic rats (P < 0.01). The mRNA expression levels of PPAR-α and LDL-R were decreased in the liver of hyperlipidemic rats compared with normal ones, and they were increased by intervention with TOAWA. Besides, the degree of hepatic steatosis was alleviated by TOAWA. Conclusion: TOAWA can decrease blood lipid levels in hyperlipoidemic rats by altering the level of apolipoprotein in serum and upregulating the mRNA expression levels of PPAR-α and LDL-R in liver and improving liver function.

Key words: total organic acids from wild apricot, hyperlipidemia, blood lipids, hypolipidemic mechanism

中图分类号:

R965.1

阿依姑丽·艾合麦提，王妙颖，彭禛菲，冯雅丽，王小燕，古扎丽努尔·艾尼. 野山杏果肉总有机酸对高脂血症大鼠血脂及相关基因表达的影响[J]. 食品科学, 2018, 39(21): 171-176.

Aygul·AHMAT, WANG Miaoying, PENG Zhenfei, FENG Yali, WANG Xiaoyan, Guzalnur·GENI. Effect of Total Organic Acid from Wild Apricot on Blood Lipid and the Expression of Related Genes in Hyperlipidemic Rats[J]. FOOD SCIENCE, 2018, 39(21): 171-176.

[1]	陈选，陈旭，韩金志，汪少芸. 海洋鱼源抗菌肽的研究进展及其在食品安全中的应用前景[J]. 食品科学, 2021, 42(9): 328-335.
[2]	詹慧，宋田源，余刚，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性[J]. 食品科学, 2021, 42(7): 142-148.
[3]	谢文，陈华国，赵超，龚小见，周欣. 枸杞多糖的生物活性及作用机制研究进展[J]. 食品科学, 2021, 42(5): 349-359.
[4]	范三红，王娇娇，李佳妮，白宝清. 辣椒红色素和辣椒碱体内抗氧化活性及降血脂功能[J]. 食品科学, 2021, 42(5): 201-207.
[5]	陈巧莉，杨兵，洪晴悦，魏鲟钰，方楚楚，阚建全. 海洋生物毒素的分类、毒害作用机制及检测技术研究进展[J]. 食品科学, 2021, 42(5): 321-331.
[6]	刘寒寒，杨书珍，李哲，张美红，彭丽桃. 碳酸铵对柑橘酸腐病菌的抑制效果及作用机制[J]. 食品科学, 2021, 42(3): 204-210.
[7]	阎然，傅茂润，陈蕾蕾，李有媛，徐敏慧，何志平，周庆新. 解淀粉芽孢杆菌NCPSJ7对采后脐橙绿霉病的防治作用及机制[J]. 食品科学, 2021, 42(17): 193-200.
[8]	高渊，杨亚茹，常明昌，孟俊龙，刘靖宇，冯翠萍. 基于代谢组学研究绣球菌多糖对高脂血症大鼠的降血脂作用[J]. 食品科学, 2021, 42(11): 168-175.
[9]	魏连会，宋淑敏，董艳，石杰，王娇娇，李宝龙，孙兴荣. 火麻籽多肽对高脂饮食喂养大鼠血脂的影响[J]. 食品科学, 2021, 42(11): 161-167.
[10]	李志春，陈赶林，郑凤锦，孙健，林波，方晓纯. 甘蔗醋对高脂小鼠血脂代谢及组织氧化还原的影响[J]. 食品科学, 2020, 41(9): 86-90.
[11]	陈梦玲，蓝蔚青，李函笑，任智楚，芦子萱，谢晶. 牛至精油对腐生葡萄球菌抑制作用机制[J]. 食品科学, 2020, 41(7): 46-51.
[12]	边亮，陈华国，周欣. 植物多糖的抗肿瘤活性研究进展[J]. 食品科学, 2020, 41(7): 275-282.
[13]	温永平，朱迪，孙健，闫巧娟，江正强. 魔芋甘露寡糖抗肥胖活性及机制[J]. 食品科学, 2020, 41(5): 115-121.
[14]	刘淑婷，王颖，王志辉，王迪，张艳莉，佐兆杭，王浩宇，邓薇. 紫花芸豆抗性淀粉对高脂血症大鼠肝脏及肠屏障损伤的修复作用[J]. 食品科学, 2020, 41(21): 106-112.
[15]	崔敬爱，王思霁，刘畅，于婷，陈晓平. 桦褐孔菌多糖对高脂饮食诱导的高脂血症大鼠血脂和肝脏的保护作用及机制[J]. 食品科学, 2020, 41(19): 185-190.

野山杏果肉总有机酸对高脂血症大鼠血脂及相关基因表达的影响

Effect of Total Organic Acid from Wild Apricot on Blood Lipid and the Expression of Related Genes in Hyperlipidemic Rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价