关键词: 离子色谱, 脉冲安培检测, 蔗糖, 葡萄糖, 果糖

Abstract: An ion chromatographic method has been developed for the determination of sucrose, glucose and fructose in two kinds of fruits and two kinds of drinks. The analytes were separated on a METROSEP CARB 1 (150 mm × 4.0 mm) anion exchange column using 30.0 mmol/L NaOH as mobile phase at flow rate of 1.0 mL/min and detected with a pulsed amperometric detector. The total time it took to separate the sugars was 25 min. The linear ranges of sucrose, glucose and fructose were 1.0－50.0 mg/L. The detection limits for sucrose, glucose and fructose were 0.085, 0.126 mg/L and 1.072 mg/L (20μL injection, and the quantitative analysis was based on the peak areas), respectively. The RSDs of peak areas for five consecutive determinations of 8 mg/L sucrose, glucose and fructose were 6.09%, 2.90% and 3.03%, respectively. The RSDs of retention time were 2.49%, 1.78% and 1.79%, respectively. The recovery rates for the sugars across three spike levels were in the range of 83.4%－126.6%. Satisfactory results were achieved for the determinations of sucrose, glucose and fructose by the method. Meanwhile, the method was simple, accurate, fast, effective and applicable for the quality control of fruits and drinks.

Key words: ion chromatography, pulsed amperometric detection, sucrose, glucose, fructose