酵母甘露糖蛋白体外发酵及其代谢产物的抗炎活性

摘要/Abstract

摘要： 酵母甘露糖蛋白（Mannoprotein, MP）具有多种生物活性，但其对肠道菌群的影响尚不清楚。本研究通过体外肠道菌群厌氧发酵技术研究MP的益生活性。结果表明，MP可调节肠道菌群结构，在门水平增加了拟杆菌门（Bacteroidetes）的相对丰度，降低了厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）的相对丰度，降低了Firmicutes/ Bacteroidetes的比值。MP显著提高了发酵液中短链脂肪酸（short-chain fatty acids, SCFAs）尤其是乙酸和丙酸的含量。MP与菊粉具有相似的益生菌活性，菊粉可促进双歧杆菌属（Bifidobacterium）的增殖，而MP可选择性促进拟杆菌属（Bacteroides）、韦荣球菌属（Veillonella）、Clostridium_sensu_srticto、布劳特氏菌属（Blautia）、粪杆菌属（Faecalibacterium）、纺锤链杆属（Fusicatenibacter）和产丁酸球菌属（Butyricicoccus）的生长。脂多糖诱导RAW 264.7巨噬细胞模型结果表明MP对炎症没有显著调节作用（p > 0.05），而其发酵液具有显著的抗炎活性，且其活性与菊粉组相似，均优于空白组。因此，MP有望成为通过调节肠道菌群来改善人体健康的功能性食品。

关键词: 酵母, 甘露糖蛋白, 肠道微生物, 短链脂肪酸, 抗炎活性

Abstract: The mannoprotein (MP) from yeast has many bioactivities. However, the effect of yeast MP on the gut microbiota is still unknown. Thus, the anaerobic fermentation of MP by gut microbiota in vitro was carried out to evaluate the probiotic activity of MP in this work. The result showed that MP could modulate the structure of gut microbiota. Furthermore, MP, increased the relative abundance of Bacteroidetes, decreased the relative abundances of Firmicutes, Proteobacteria, and the ratio of Firmicutes to Bacteroidetes. The concentration of short-chain fatty acids, especially acetic acid and propionic acid, were significantly increased by MP. The MP showed a similar probiotic activity with inulin. Differently, inulin could stimulate the proliferation of Bifidobacterium, whereas MP could selectively promote the growths of Bacteroides, Veillonella, Clostridium_sensu_srticto, Blautia, Faecalibacterium, Fusicatenibacter and Butyricicoccus. The cell model of RAW 264.7 macrophage cells induced by lipopolysaccharide (LPS) showed that MP had limited anti-inflammatory effect (p > 0.05). Whereas, the fermentation solution of MP showed similar significant anti-inflammatory activity with that of inulin, which was superior to that of blank group. Thus, MP was expected to serve as functional food for improvement of our health by modulating gut microbiota.

Key words: Yeast, mannoprotein, gut microbiota, Short-Chain Fatty Acids (SCFAs), anti-inflammatory activity

中图分类号:

TS201.4

李祥陈贵杰康贻军 KHANDAN Amirsalar. 酵母甘露糖蛋白体外发酵及其代谢产物的抗炎活性[J]. 食品科学, 0, (): 0-0.

xiang li. Fermentation of yeast mannoprotein in vitro and anti-inflammatory activity of its metabolites[J]. FOOD SCIENCE, 0, (): 0-0.

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