食品科学 ›› 2010, Vol. 31 ›› Issue (18): 143-146.doi: 10.7506/spkx1002-6630-201018033

• 工艺技术 • 上一篇    下一篇

复合酶法提取红蘑多糖工艺

李书倩1,2,张 博1,辛 广1,于 洋1,刘长江2,*   

  1. 1.鞍山师范学院化学系 2.沈阳农业大学食品学院
  • 收稿日期:2010-06-07 修回日期:2010-07-27 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 刘长江 E-mail:liucj597@sohu.com
  • 基金资助:

    鞍山科技计划项目(08SF24)

Double-enzyme Method for Polysaccharides Extraction from Gomphidius rutilus Fruitbodies

LI Shu-qian1,2,ZHANG Bo1,XIN Guang1,YU Yang1,LIU Chang-jiang2,*   

  1. 1. Department of Chemistry, Anshan Normal University, Anshan 114007, China;2. College of Food, Shenyang Agricultural University, Shenyang 110161, China
  • Received:2010-06-07 Revised:2010-07-27 Online:2010-09-25 Published:2010-12-29
  • Contact: LIU Chang-jiang2,* E-mail:liucj597@sohu.com

摘要:

以野生红蘑为原料,复合酶法浸提红蘑中真菌多糖。在单因素试验的基础上,采用正交试验设计获得最佳多糖提取工艺,即酶解温度60℃、纤维素酶和木瓜蛋白酶的比例为3:1(m/m)、pH5.0、酶解3h,此条件下提取多糖得率为12.0%。与传统方法相比,该工艺具有提取条件温和,提取时间较短,对多糖损伤小等特点。

关键词: 红蘑, 多糖, 提取, 复合酶

Abstract:

For polysaccharide extraction, wild Gomphidius rutilus fruit bodies were hydrolyzed with cellulase and papain together. Based on single factor investigations, orthogonal array design was employed to optimize the hydrolysis process, and the optimum hydrolysis conditions were found as follows: hydrolysis temperature 60 ℃; cellulase/papain mass ratio 3:1; pH 5.0; and hydrolysis duration 3 h and the polysaccharide yield obtained under such conditions was 12.0%. This method has many advantages over conventional methods, such as gentle reaction conditions, time saving and less destructive effect on polysaccharide structure.

Key words: Gomphidius rutilus, polysaccharide, extraction, compound enzymes

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