Abstract: Taking space mutation treatment Cordyceps militaris and its the primitive strain as a material,determines the cordycepin and adenosine with HPLC,determines the cordyceps acid with periodic acid sodium colorimetry method.The phenol-sulphuric acid method was used in the polysaccharides content.To analysis the change of active constituent content with space mutation treatment.Cordycepin content of space mutation treatment Cordyceps militaris was 2.5 times of primitive strain.At the same time the adenosine content was enhanced 2 times,the active constituent total quantity enhanced 26.2 %.Space mutation treatment enhances the Cordyceps militaris the active constituent content.

Key words: Cordyceps militaris, mutation breeding, cordycepin, adenosine, polysaccharide, cordyceps acid