食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 99-101.doi: 10.7506/spkx1002-6300-200918018

• 工艺技术 • 上一篇    下一篇

香薷多糖的提取与纯化研究

杨 超,李景恩,邱增辉,聂少平* ,殷军艺,李 昌,谢明勇   

  1. 南昌大学 食品科学与技术国家重点实验室
  • 收稿日期:2009-04-28 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 聂少平 E-mail:spnie@ncu.edu.cn
  • 基金资助:

    食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200806);教育部“长江学者和创新团队发展计划”项目(IRT0540);大学生创新性实验项目资助项目(081040306)

Extraction and Purification of Polysaccharides from Herba Moslae

YANG Chao,LI Jing-en,QIU Zeng-hui,NIE Shao-ping*,YIN Jun-yi,LI Chang,XIE Ming-yong   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2009-04-28 Online:2009-09-15 Published:2010-12-29
  • Contact: NIE Shao-ping E-mail:spnie@ncu.edu.cn

摘要:

利用热水浸提、乙醇醇沉提取水溶性香薷粗多糖,脱蛋白、除色素后所得的精制多糖经DEAE-52 纤维素阴离子交换色谱得两个组分Ⅰ和Ⅱ。主要组分Ⅱ经Sephacryl S-300 葡聚糖凝胶色谱进一步纯化,得单一多糖组分,命名为HMP Ⅱ。经高效凝胶渗透色谱检测其纯度为93.55%。

关键词: 香薷多糖, 提取, 纯化

Abstract:

Crude water-soluble polysaccharides were isolated from Herba Moslae by hot water extraction and ethanol precipitation. After protein and pigment removal, the refined polysaccharides were separated by DEAE-52 cellulose anionexchange chromatography, and two fractions Ⅰand Ⅱ were obtained. The main fraction Ⅱ was further purified by Sephacryl S-300 gel chromatography and a polysaccharide named HMPⅡ was obtained. The purity of HMPⅡ was determined to be 93.55% by high performance gel permeation chromatography (HPGPC).

Key words: polysaccharides from Herba Moslae, extraction, purification

中图分类号: