食品科学 ›› 2009, Vol. 30 ›› Issue (20 ): 347-349.doi: 10.7506/spkx1002-6300-200920077

• 分析检测 • 上一篇    下一篇

独根草不同部位多糖含量的测定

李文芳,晏 丽,银 伟,李 望   

  1. 吉首大学城乡资源与规划学院
  • 收稿日期:2009-07-22 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 李文芳

Determination of Polysaccharides in Different Parts of Oresitrophe rupifraga Bge

LI Wen-fang,YAN Li,YIN Wei,LI Wang   

  1. College of Resources of Planning Sciences, Jishou University, Zhangjiajie 427000, China
  • Received:2009-07-22 Online:2009-10-15 Published:2010-12-29
  • Contact: LI Wen-fang

摘要:

采用水提-醇沉法对独根草根、茎、叶中的有效成分多糖进行提取,经蒽酮-硫酸显色后,在624nm波长处比色测定。结果独根草根、茎、叶中的多糖含量分别为6.28%、10.47%、3.29%。结果表明独根草茎是独根草全株以多糖为主的重要活性部位。

关键词: 独根草, 多糖, 测定

Abstract:

Bioactive components of polysaccharides were extracted from roots, stems, and leaves of Oresitroph rupifraga Bge using water extraction-ethanol precipitation. Meanwhile, the contents of polysaccharides were determined at 624 nm by anthrone-sulfuric acid method. The contents of polysaccharides in roots, stems and leaves of Oresitrophe rupifraga Bge were 6.28%, 10.47%, and 3.29%, respectively. These results indicated that stems of Oresitrophe rupifraga Bge are the major locations of bioactive polysaccharides.

Key words: Oresitrophe rupifraga Bge, polysaccharide, determination

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