食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 237-240.doi: 10.7506/spkx1002-6300-200921056

• 生物工程 • 上一篇    下一篇

沙城龙眼葡萄自然发酵过程相关酵母生物多样性研究

李 艳1,2,卢 君1,张利中3,崔建东1,2   

  1. 1.河北科技大学生物科学与工程学院 2.河北省发酵工程技术研究中心 3.中国长城葡萄酒有限公司
  • 收稿日期:2009-06-20 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 李艳 E-mail:Lymdh5885@163.com

Yeast Biodiversity in Longan Grapes during Spontaneous Fermentation Process

LI Yan1,2,LU Jun1,ZHANG Li-zhong3,CUI Jian-dong1,2   

  1. 1. College of Bioscience and Bioengineering , Hebei University of Science and Technology, Shijiazhuang 050018, China;
    2. R&D Center for Fermentation Engineering of Hebei Province, Shijiazhuang 050018, China;
    3. China Great Wall Wine Co. Ltd., Shacheng 075400, China
  • Received:2009-06-20 Online:2009-11-01 Published:2010-12-29
  • Contact: LI Yan E-mail:Lymdh5885@163.com

摘要:

本实验从沙城产区龙眼葡萄自然发酵过程中分离得到120 株酵母菌株,利用WL 培养基进行形态聚类分析,得到5 种类型,再通过5.8S rDNA-ITS 区域RFLP 的方法得到5 种不同的酶切图谱,最后经5.8S rDNA-ITS 区域序列分析,将4 种类型的酵母鉴定到种的水平,另外一个类型的酵母需要进一步的鉴定。

关键词: 龙眼葡萄, 自然发酵, 酵母, 生物多样性

Abstract:

Totally 120 yeast strains were isolated from longan grapes harvested from Shacheng region during spontaneous fermentation process. Five unique colonies were identified through culture and clustering analysis using WL medium. Five different genotypes were also identified through RFLP analysis of 5.8S rDNA-ITS region. The species of four yeast strains were identified through sequence analysis and another one still needs to be further identified.

Key words: longan grape, spontaneous fermentation, yeast, biodiversity

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