食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 294-296.doi: 10.7506/spkx1002-6300-200921067

• 生物工程 • 上一篇    下一篇

葛粉双歧杆菌发酵饮料发酵条件的研究

顾仁勇,银永忠   

  1. 吉首大学化学化工学院食品科学与工程系
  • 收稿日期:2009-07-22 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 顾仁勇 E-mail:gry8565398@163.com
  • 基金资助:

    湖南省科技厅科技计划一般项目(2008NK3112)

Optimization of Fermentation Condition for Kudzu Beverage Production by Bifidobacterium

GU Ren-yong,YIN Yong-zhong   

  1. Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Jishou
    University, Jishou 416000, China
  • Received:2009-07-22 Online:2009-11-01 Published:2010-12-29
  • Contact: GU Ren-yong E-mail:gry8565398@163.com

摘要:

以两歧双歧杆菌、嗜热链球菌和保加利亚乳杆菌(1:1:1 混合)为发酵菌种,采用单因素试验和正交试验对葛粉发酵饮料的发酵条件进行优化。优选出的发酵条件为:葛粉乳液中添加牛乳70%、葡萄糖1.5%、蔗糖5.0%、混合菌种接种量6.0%、发酵温度40℃、发酵时间10h。在此条件下产品发酵酸度为5.20%,活菌数7.14 × 107CFU/ml,口感良好。

关键词: 两歧双歧杆菌, 葛粉, 发酵

Abstract:

A fermented kudzu beverage was developed using the starter composed of Bifidobacterium, Thermophil streptocoaus and Bulgaria lactobacillaceae with a ratio of 1:1:1. The optimal fermentation process for kudzu beverage production was determined to be fermentation for 10 h at 40 ℃ with an inoculation size of 6.0% using kudzu milk solution with 70% milk and 1.5% glucose as fermentation substrate by one-factor-at-a-time and orthogonal array design methods. The acidity and total amount of viable Bifidobacteria in products fermented under these optimal conditions were 0.52% and 7.14 × 107 CFU/ml. Meanwhile, the obtained products exhibited a nice taste.

Key words: Bifidobacterium, kudzu, fermentation

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