食品科学 ›› 2009, Vol. 30 ›› Issue (21): 316-319.doi: 10.7506/spkx1002-6300-200921073

• 生物工程 • 上一篇    下一篇

基因组改组鼠李糖乳杆菌生产L-乳酸发酵培养基的优化

王玉华1,陈 萍1,朴春红1,王 磊2   

  1. 1.吉林农业大学食品工程学院 2.长春市水务集团
  • 收稿日期:2008-08-21 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 王玉华 E-mail:wangyuhua@jlu.edu.cn
  • 基金资助:

    吉林省农业科技发展计划项目(200302-02)

Optimization of Fermentation Medium Composition for L-lactic Acid Production by Genome-shuffled Lactobacillus rhamnosus

WANG Yu-hua1,CHEN Ping1,PIAO Chun-hong1,WANG Lei2   

  1. 1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China ;
    2. Changchun Water Group, Changchun 130109, China
  • Received:2008-08-21 Online:2009-11-01 Published:2010-12-29
  • Contact: WANG Yu-hua E-mail:wangyuhua@jlu.edu.cn

摘要:

通过单因素试验确定了耐酸性强、高产L- 乳酸的改组菌株Lc-F34 的发酵最适碳源为葡萄糖、初始葡萄糖浓度为90~110g/L,淀粉葡萄糖水解物可以作为乳酸发酵的碳源;最适氮源为酵母抽提物,玉米浆次之,玉米浆可以部分或全部代替酵母抽提物作为乳酸发酵的氮源;适量的MgSO4 和MnSO4 对该菌株的发酵有刺激作用,MgSO4 最适浓度为0.3%,MnSO4 最适浓度为0.05%。

关键词: 鼠李糖乳杆菌, L - 乳酸, 发酵培养基, 优化

Abstract:

The optimal carbon source in the fermentation medium for L-lactic acid production by an acid-resistant and high L-lactic acid-producing strain (Lc-F34) of genome-shuffled Lactobacillus was determined to be glucose with an initial concentration range of 90-110 g/L by one-factor-at-a-time method and starch hydrolysates also could be used as carbon source. In addition, the optimal nitrogen source was determined to be yeast extract, followed by corn steep liquor, which was able to partially or completely replace yeast extract. Furthermore, moderate addition of MgSO4·7H2O or MnSO4 could stimulate L-lactic acid fermentation with optimal concentrations of 0.3% and 0.05% respect to each.

Key words: Lactobacillus rhamnosus, L-lactic acid, fermentation medium, optimization

中图分类号: